CORE--ADVANCED CELL TECHNOLOGIES

核心——先进的细胞技术

• 批准号: 6316584
• 负责人: LOLA M REID
• 金额: $ 16.67万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6316584
• 关键词: biomedical facility; cell line; extracellular matrix; gastrointestinal disorder; gastrointestinal function; gastrointestinal system; tissue /cell culture

DESCRIPTION (Adapted from the application) This core was established at the time of the last competing renewal. This core will help the investigators with ex vivo maintenance of highly differentiated cells and tissues. The specific aims are to provide: 1.a facility with state-of-the-art expertise and equipment with which to perform sophisticated forms of cell culture; 2. stocks of highly differentiated cell lines; 3. tested reagents such as collagenase and serum tested for efficacy on liver and GI tissues; 4. preparations of extracellular matrix components of tissue extracts enriched in ECM and/or assistance with utilization of commercially available forms of ECM components; 5. consultation and assistance with designs for optimal culture conditions for ex vivo maintenance of differentiated cell types whether primary or for cell lines; 6. serum-free chemically and hormonally defined media tailored for growth or for differentiation of liver or GI tissue-derived cells or for cell lines; 7. freshly isolated cell suspensions from normal or diseased tissues of liver or GI tract; and 8. assistance with sophisticated forms of flow cytometry for isolation of and/or characterization of cells derived from the liver or GI tissue.

描述(改编自应用程序)此核心建立在 上次竞争续订的时间。这个核心将帮助调查人员 在体外维持高度分化的细胞和组织。具体的 目标是提供：1.拥有最先进的专业知识和设备的设施 用来进行复杂形式的细胞培养；2.高度 分化细胞系；3.胶原酶和血清等检测试剂 对肝脏和胃肠道组织的疗效测试；4.细胞外制剂 富含ECM和/或辅助的组织提取物的基质成分 利用市面上可获得的ECM组件形式；5.协商 并协助设计最佳的体外培养条件 保持分化的细胞类型，无论是原代细胞还是细胞系； 无血清化学和激素定义的培养基量身定做，适合生长或 肝或胃肠道组织来源的细胞或细胞系的分化； 新鲜分离的正常或病变肝组织细胞悬液 胃肠道；以及8.协助复杂形式的流式细胞术 肝或胃肠道来源细胞的分离和/或鉴定 组织。