CORE--ADVANCED CELL TECHNOLOGIES

核心——先进的细胞技术

• 批准号: 6410310
• 负责人: LOLA M REID
• 金额: $ 16.67万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-12-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6410310
• 关键词: biomedical facility; cell line; extracellular matrix; gastrointestinal disorder; gastrointestinal function; gastrointestinal system; tissue /cell culture

DESCRIPTION (Adapted from the application) This core was established at the time of the last competing renewal. This core will help the investigators with ex vivo maintenance of highly differentiated cells and tissues. The specific aims are to provide: 1.a facility with state-of-the-art expertise and equipment with which to perform sophisticated forms of cell culture; 2. stocks of highly differentiated cell lines; 3. tested reagents such as collagenase and serum tested for efficacy on liver and GI tissues; 4. preparations of extracellular matrix components of tissue extracts enriched in ECM and/or assistance with utilization of commercially available forms of ECM components; 5. consultation and assistance with designs for optimal culture conditions for ex vivo maintenance of differentiated cell types whether primary or for cell lines; 6. serum-free chemically and hormonally defined media tailored for growth or for differentiation of liver or GI tissue-derived cells or for cell lines; 7. freshly isolated cell suspensions from normal or diseased tissues of liver or GI tract; and 8. assistance with sophisticated forms of flow cytometry for isolation of and/or characterization of cells derived from the liver or GI tissue.

描述（根据应用程序改编）该核心是在 最后一次竞争续约的时间。该核心将帮助调查人员 离体维持高度分化的细胞和组织。具体 目的是提供：1.a具有最先进的专业知识和设备的设施 用它执行细胞培养的复杂形式； 2。高度股票 分化的细胞系； 3。经过测试的试剂，例如胶原酶和血清 测试了肝脏和胃肠道组织的功效； 4。细胞外的准备 组织提取物的基质成分富含ECM和/或辅助 利用市售形式的ECM组件； 5。咨询 以及为实体的最佳文化条件设计的帮助 维持分化的细胞类型，无论是主要的还是细胞系； 6。 无血清化学和荷尔蒙定义的培养基，适合生长或 肝或胃肠道衍生细胞或细胞系的分化； 7。 来自肝脏正常或患病组织的新鲜分离细胞悬浮液或 胃肠道； 8。以复杂形式的流式细胞仪的帮助 源自肝脏或GI的细胞的分离和/或表征 组织。