BIOCHEMISTRY OF EUKARYOTIC MESSENGER RNA SYNTHESIS

描述（改编自申请人的摘要）：本项目的长期目标 研究旨在了解调节的生化机制 真核生物的mRNA合成。具体而言，研究侧重于了解 哺乳动物RNA聚合酶II的活性是如何被 转录因子指导转录物的起始和延伸， 大量的核心启动子。总的办法是 用纯化的酶重组，忠实而有效地转录， 体外，并使用此重建系统来阐明生物化学 哺乳动物RNA转录起始和延伸的调控机制 聚合酶II。本研究将沿着以下路线组织：（1） 详细分析新型RNA聚合酶II的作用机制 延伸因子ELL和ELL 2。(2)进一步研究了 RNA聚合酶II延伸因子Elongin的作用。(3)纯化及 RNA聚合酶II延伸因子S-IV的表征。(4)详细 TFIIE、TFIIF、TFIIH和ATP在转录中作用的研究 启动和有效启动子逃逸。此外，本发明还 建议继续研究功能关系 在延伸蛋白B和C调节亚基与VHL的产物之间 肿瘤抑制基因

DESCRIPTION(adapted from applicant's abstract): The long-term goal of this study is to understand the biochemical mechanisms that underlie the regulation of eukaryotic mRNA synthesis. Specifically, the studies focus on understanding how the activity of the mammalian RNA polymerase II is controlled by transcription factors that direct initiation and elongation of transcripts from a large number of core promoters in vitro. The overall approach is to reconstitute with purified enzymes, faithful and efficient transcription in vitro, and to use this reconstituted system to elucidate the biochemical mechanisms governing transcription initiation and elongation by mammalian RNA polymerase II. The research will be organized along the following lines: (1) Detailed analysis of the mechanisms of action of novel RNA polymerase II elongation factors ELL and ELL2. (2) Further investigation of the mechanism of action of RNA polymerase II elongation factor Elongin. (3) Purification and characterization of RNA polymerase II elongation factor S-IV. (4) Detailed Investigation of the roles of TFIIE, TFIIF, TFIIH and ATP in transcription initiation and efficient promoter escape by RNA polymerase II. Additionally, studies are proposed to continue the studies on the functional relationship between the Elongin B and C regulatory subunits and the product of the VHL tumor suppressor gene.