TRANSCRIPTION OF ACANTHAMOEBA RNA PLYMERASE II GENES

描述（改编自申请人的摘要）：卡氏阿米巴 是一个小的，自由生活的变形虫，是病原体的阿米巴原虫 角膜炎。阿米巴可以支持几种人类细胞的细胞内生长， 病原体并且可能代表细菌的重要环境储存库， 其需要细胞内生长。阿米巴的特征是有丝分裂 生长周期，并在不利条件下形成包囊 遇到了。分化的分子机制主要是 未知这是一个建议，以表征基因表达的机制， 生长和分化。两个对生长至关重要的基因，TBP基因和 调节它的因子TPBF的基因将被表征为： 体外和体内。编码一种转录调节的， 特异性蛋白质（CSP21）已被克隆，提供了机会， 表征细胞特异性基因表达的机制。

DESCRIPTION (Adapted from the Applicant's Abstract): Acanthamoeba castellanii is a small, free living amoeba that is the causative agent for Acanthamoeba keratitis. Acanthamoeba can support intracellular growth for several human pathogens and may represent a significant environmental reservoir for bacteria, which require intracellular growth. Acanthamoeba is characterized by a mitotic growth cycle, and formation of an encapsulated cyst when unfavorable conditions are encountered. The molecular mechanisms of differentiation are largely unknown. This is proposal to characterize mechanisms of gene expression during growth and differentiation. Two genes essential for growth, the TBP gene and the gene of the factor, which regulates it, TPBF, will be characterized in vitro and in vivo. The gene encoding a transcriptionlly regulated, cyst specific protein (CSP21) has been cloned, providing an opportunity to characterize the mechanisms of cell-specific gene expression.