未耕种土壤细菌的功能基因组学-猫眼课题宝

未耕种土壤细菌的功能基因组学

批准号：

6207548

负责人：

MARK R LILES

金额：

$3.75万

依托单位：

UNIVERSITY OF WISCONSIN-MADISON

依托单位国家：

美国

项目类别：

财政年份：

2000

资助国家：

美国

项目状态：

未结题

起止时间：

2000-07-01 至

来源：

https://reporter.nih.gov/project-details/6207548

关键词：

Escherichia coli Rhodospirillales antibiotics bacteria bacterial genetics drug discovery /isolation functional /structural genomics genetic library green fluorescent proteins microorganism culture molecular cloning soil microbiology soil sampling transfection /expression vector

中文摘要

翻译

通过使用分子技术评估微生物多样性，揭示了生物世界的新前景。尽管有了这一发现，整个部门级别的细菌群体仍然存在于自然环境中，这些环境难以培养，可能含有以前未发现的抗生素。这项研究将获得新的酸杆菌科内的功能多样性，为发现抗生素提供依据。利用这个部门中来自细菌的基因组克隆的集合，将开发出一个异源表达系统。通过根据G-C含量分离土壤基因组DNA，富含G-C含量的DNA组分和富含酸杆菌基因组的DNA组分将被用于广泛寄主范围的粘粒基因组文库的构建。对这种富含酸性细菌的基因组文库进行筛选，以寻找对重要人类病原体具有活性的克隆，可能会导致分离出新的抗生素，其遗传决定因素和化学结构将被确定。

英文摘要

New vistas in the biological world have been revealed through the use of molecular techniques for assessing microbial diversity. Despite this revelation, whole division-level groups of bacteria exist in natural environments that are refractory to cultivation and potentially harbor previously undiscovered antibiotics. This study will access the functional diversity within the novel division Acidobacterium for the discovery of antibiotic agents. Using a collection of genomic clones derived from bacteria within this division, a heterologous expression system will be developed. By separating soil genomic DNA on the basis of G+C content, DNA fractions enriched for the presence of G+C content, DNA fractions enriched for the presence of Acidobacterium genomes will be used for genomic library construction in a broad host-range cosmid. Screening of this Acidobacterium-enriched genomic library for clones with activity against important human pathogens may result in the isolation of novel antibiotic agents, the genetic determinants and chemical structure of which will be determined.

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