FUNCTIONAL GENOMICS OF UNCULTIVATED SOIL BACTERIA

未耕种土壤细菌的功能基因组学

• 批准号: 6385223
• 负责人: MARK R LILES
• 金额: $ 4.2万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6385223
• 关键词: Escherichia coli; Rhodospirillales; antibiotics; bacteria; bacterial genetics; drug discovery /isolation; functional /structural genomics; genetic library; green fluorescent proteins; microorganism culture; molecular cloning; soil microbiology; soil sampling; transfection /expression vector

New vistas in the biological world have been revealed through the use of molecular techniques for assessing microbial diversity. Despite this revelation, whole division-level groups of bacteria exist in natural environments that are refractory to cultivation and potentially harbor previously undiscovered antibiotics. This study will access the functional diversity within the novel division Acidobacterium for the discovery of antibiotic agents. Using a collection of genomic clones derived from bacteria within this division, a heterologous expression system will be developed. By separating soil genomic DNA on the basis of G+C content, DNA fractions enriched for the presence of G+C content, DNA fractions enriched for the presence of Acidobacterium genomes will be used for genomic library construction in a broad host-range cosmid. Screening of this Acidobacterium-enriched genomic library for clones with activity against important human pathogens may result in the isolation of novel antibiotic agents, the genetic determinants and chemical structure of which will be determined.

通过使用分子技术评估微生物多样性，揭示了生物界的新前景。尽管有这一发现，整个部门级的细菌群体存在于难以培养的自然环境中，并且可能含有以前未发现的抗生素。本研究将探讨新分支酸杆菌的功能多样性，以发现抗生素。使用来自该部门内的细菌的基因组克隆的集合，将开发异源表达系统。通过基于G+C含量分离土壤基因组DNA，富集G+C含量的DNA级分、富集酸杆菌基因组的DNA级分将用于在宽宿主范围粘粒中构建基因组文库。筛选这种富含酸杆菌的基因组文库中具有抗重要人类病原体活性的克隆可能导致分离出新型抗生素制剂，其遗传决定簇和化学结构将被确定。