TRANSPEPTIZYME INDUCED PEPTIDE BOND FORMATION

该计划的长期目标是了解蛋白质合成的演变。 具体来说，拟议的研究旨在基于tRNA的受体茎环来确定氨酰-RNA系统的日期，这将允许以非编码方式形成肽键。 这种氨酰-RNA复合物可能是一种原始的蛋白质翻译系统。 由RNA编码的遗传信息被翻译成蛋白质，蛋白质是细胞中几乎所有生化和结构功能的核心。 因此，分析肽合成的基本过程可能有助于更好地了解人类疾病的起源。 将用于实现所提出的目标的两种策略是1）利用基于P4-P6四膜虫核酶复合物的结构的可氨酰化的RA支架来构建肽，以及2）开发由发夹微螺旋和单链核糖核苷酸组成的氨酰化RNA三螺旋系统来产生肽。

The long-term objective of this proposal is to understand the evolution of protein synthesis. Specifically, the proposed research is designed to date aminoacyl-RNA systems based on the acceptor stem loop of tRNAs that will allow for peptide bond formation in a noncoded fashion. This aminoacyl-RNA complex may have served as a primitive protein translation system. The genetic information encoded by RNA is translated into proteins, which are central to virtually every biochemical and structural function in cells. Accordingly, analysis of the basic process of peptide synthesis may contribute to a greater understanding of the origins of human diseases. The two strategies that will be employed to achieve the proposed goal are 1) utilize an aminoacylatable RA scaffold based on the structure of the P4-P6 tetrahymena ribozyme complex to construct peptides and 2) develop an aminoacylated RNA triple helix system consisting of a hairpin minihelix and a single-stranded ribonucleotide to generate peptides.