TRANSPEPTIZYME INDUCED PEPTIDE BOND FORMATION

转肽酶诱导肽键形成

• 批准号: 6385171
• 负责人: ANIL T ABRAHAM
• 金额: $ 0.57万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6385171
• 关键词: Tetrahymena; aminoacyl tRNA; biochemical evolution; chemical bond; genetic translation; peptide chemical synthesis; ribozymes; transpeptidation

The long-term objective of this proposal is to understand the evolution of protein synthesis. Specifically, the proposed research is designed to date aminoacyl-RNA systems based on the acceptor stem loop of tRNAs that will allow for peptide bond formation in a noncoded fashion. This aminoacyl-RNA complex may have served as a primitive protein translation system. The genetic information encoded by RNA is translated into proteins, which are central to virtually every biochemical and structural function in cells. Accordingly, analysis of the basic process of peptide synthesis may contribute to a greater understanding of the origins of human diseases. The two strategies that will be employed to achieve the proposed goal are 1) utilize an aminoacylatable RA scaffold based on the structure of the P4-P6 tetrahymena ribozyme complex to construct peptides and 2) develop an aminoacylated RNA triple helix system consisting of a hairpin minihelix and a single-stranded ribonucleotide to generate peptides.

这项提议的长期目标是了解蛋白质合成的进化。具体地说，这项拟议的研究旨在确定基于tRNAs的受体茎环的氨酰-RNA系统的日期，该系统将允许以非编码方式形成肽键。这种氨基酰-RNA复合体可能是一种原始的蛋白质翻译系统。由RNA编码的遗传信息被翻译成蛋白质，蛋白质是细胞中几乎所有生化和结构功能的核心。因此，分析多肽合成的基本过程可能有助于更好地了解人类疾病的起源。为实现所提出的目标，将采用两种策略：1)利用基于P4-P6四膜虫核酶复合体结构的氨基酰化RA支架构建多肽；2)开发由发夹微螺旋和单链核苷酸组成的氨基酰化RNA三螺旋系统来生成肽。