MECHANISM OF CELL DIVISION PLANE PLACEMENT IN S POMBE

S Pombe 中细胞分裂平面的放置机制

• 批准号: 6056019
• 负责人: PHONG T TRAN
• 金额: $ 3.24万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6056019
• 关键词: Schizosaccharomyces pombe; cell component structure /function; cell cycle; cell nucleus; digital imaging; fungal genetics; gene targeting; lasers; microorganism culture; microtubules; protoplasm motility

Cell division is a fundamental cellular process about which little is known at the molecular level. Defects in cell division may lead to cancer and other diseases. The aim of this proposal is to use a combination of molecular genetics and biophysical approaches to answer the question: How does a cell know where to divide? The model of choice for this proposal is S. pombe, a single-cell eukaryote with simple cytoskeletal architecture, powerful genetics, and amenability for live cell imaging. In S. pombe the position of the division plane appears to be dictated by the position of the nucleus. Furthermore, nuclear positioning at the cell center is microtubule dependent. This proposal aims to use biophysical (e.g., UV ablation, high-resolution multi-mode digital microscopy, laser cutting, etc ...) and molecular genetic (e.g., gene knockout, mutant phenotype analysis, GFP-tagging, etc ...) approaches to: I) determine how the nucleus and/or the microtubules may produce forces that position the nucleus; (II) determine the function of a novel S. pombe gene, rsp1, that participates in microtubule organization and nuclear positioning.

细胞分裂是一个基本的细胞过程，在分子水平上知之甚少。 细胞分裂的缺陷可能导致癌症和其他疾病。 该提案的目的是使用分子遗传学和生物物理方法的组合来回答这个问题：细胞如何知道在哪里分裂？ 该建议的选择模型是S。粟酒裂殖酵母是一种单细胞真核生物，具有简单的细胞骨架结构、强大的遗传学和适于活细胞成像。 In S.裂面的位置似乎由核的位置决定。 此外，细胞核在细胞中心的定位是微管依赖性的。该提案旨在利用生物物理学（例如，UV烧蚀、高分辨率多模数字显微镜、激光切割等）和分子遗传学（例如，基因敲除、突变体表型分析、GFP标记等）办法：确定细胞核和/或微管如何产生使细胞核定位的力; pombe基因，rsp 1，参与微管组织和核定位。