MECHANISM OF CELL DIVISION PLANE PLACEMENT IN S POMBE

细胞分裂是一个基本的细胞过程，在分子水平上对其知之甚少。细胞分裂缺陷可能导致癌症和其他疾病。这个提议的目的是结合分子遗传学和生物物理学的方法来回答这个问题：细胞是如何知道在哪里分裂的？本研究选择的模型是S. pombe，这是一种单细胞真核生物，具有简单的细胞骨架结构、强大的遗传学和对活细胞成像的适应性。在S. pombe中，分裂面的位置似乎是由细胞核的位置决定的。此外，细胞核在细胞中心的定位依赖于微管。本提案旨在使用生物物理学（例如，紫外线消融，高分辨率多模式数字显微镜，激光切割等…）和分子遗传学（例如，基因敲除，突变表型分析，gfp标记等…）方法来：1)确定细胞核和/或微管如何产生定位细胞核的力；（II）确定一种新的S. pombe基因rsp1的功能，该基因参与微管组织和核定位。

Cell division is a fundamental cellular process about which little is known at the molecular level. Defects in cell division may lead to cancer and other diseases. The aim of this proposal is to use a combination of molecular genetics and biophysical approaches to answer the question: How does a cell know where to divide? The model of choice for this proposal is S. pombe, a single-cell eukaryote with simple cytoskeletal architecture, powerful genetics, and amenability for live cell imaging. In S. pombe the position of the division plane appears to be dictated by the position of the nucleus. Furthermore, nuclear positioning at the cell center is microtubule dependent. This proposal aims to use biophysical (e.g., UV ablation, high-resolution multi-mode digital microscopy, laser cutting, etc ...) and molecular genetic (e.g., gene knockout, mutant phenotype analysis, GFP-tagging, etc ...) approaches to: I) determine how the nucleus and/or the microtubules may produce forces that position the nucleus; (II) determine the function of a novel S. pombe gene, rsp1, that participates in microtubule organization and nuclear positioning.