SEQUENCE SPECIFIC BINDING OF DNA BY A NOVEL COILED COIL

新型卷曲线圈对 DNA 的序列特异性结合

• 批准号: 6056028
• 负责人: KEVIN M GUCKIAN
• 金额: $ 3.09万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6056028
• 关键词: DNA binding protein; DNA footprinting; circular dichroism; peptide chemical synthesis; protein binding; protein engineering; protein structure

DESCRIPTION The sequence specific recognition of DNA by proteins is extremely important for many biological processes. Due to its importance new motifs which sequence specifically recognize DNA are useful in the design of therapeutics as well as in the construction of new proteins. This proposal outlines the design of a novel motif for the sequence specific recognition of DNA and its subsequent use in the design of a DNA dependent peptide ligase. This motif consists of a heterodimeric coiled coil in which one of the solvent exposed faces is design to recognize the major groove of the AP-1 site.

描述 蛋白质对 DNA 的序列特异性识别对于许多生物过程极其重要。由于其重要性，序列特异性识别 DNA 的新基序可用于治疗药物的设计以及新蛋白质的构建。该提案概述了用于 DNA 序列特异性识别的新基序的设计及其随后在 DNA 依赖性肽连接酶设计中的应用。该基序由异二​​聚体卷曲线圈组成，其中暴露于溶剂的面之一被设计为识别 AP-1 位点的主沟。