SEQUENCE SPECIFIC BINDING OF DNA BY A NOVEL COILED COIL

蛋白质对DNA的序列特异性识别在许多生物过程中都是极其重要的。由于DNA序列的重要性，识别DNA的新基序在治疗设计和构建新的蛋白质中都是有用的。这项建议概述了一种新的序列特异性识别DNA的基序的设计以及它在设计DNA依赖的多肽连接酶中的应用。这个基序由一个异二聚体螺旋组成，其中一个暴露在溶剂中的面被设计成识别AP-1位点的主要凹槽。

DESCRIPTION The sequence specific recognition of DNA by proteins is extremely important for many biological processes. Due to its importance new motifs which sequence specifically recognize DNA are useful in the design of therapeutics as well as in the construction of new proteins. This proposal outlines the design of a novel motif for the sequence specific recognition of DNA and its subsequent use in the design of a DNA dependent peptide ligase. This motif consists of a heterodimeric coiled coil in which one of the solvent exposed faces is design to recognize the major groove of the AP-1 site.