SEQUENCE SPECIFIC BINDING OF DNA BY A NOVEL COILED COIL

描述蛋白质对DNA的序列特异性识别对于许多生物过程是极其重要的。由于其重要性，序列特异性识别DNA的新基序在治疗剂的设计以及新蛋白质的构建中是有用的。该建议概述了一种新的序列特异性识别DNA的基序的设计及其随后在DNA依赖性肽连接酶的设计中的应用。该基序由异二聚体卷曲螺旋组成，其中溶剂暴露面之一被设计为识别AP-1位点的大沟。

DESCRIPTION The sequence specific recognition of DNA by proteins is extremely important for many biological processes. Due to its importance new motifs which sequence specifically recognize DNA are useful in the design of therapeutics as well as in the construction of new proteins. This proposal outlines the design of a novel motif for the sequence specific recognition of DNA and its subsequent use in the design of a DNA dependent peptide ligase. This motif consists of a heterodimeric coiled coil in which one of the solvent exposed faces is design to recognize the major groove of the AP-1 site.