MOLECULAR MECHANISM AND DEVELOPMENT CONSEQUENCES OF ICSI

ICSI 的分子机制和发展后果

基本信息

  • 批准号:
    6151169
  • 负责人:
  • 金额:
    $ 23.37万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1999
  • 资助国家:
    美国
  • 起止时间:
    1999-02-01 至 2004-01-31
  • 项目状态:
    已结题

项目摘要

The practice of Assisted Reproductive Technology (ART) to overcome infertility in humans has increased dramatically during the past two decades and has culminated in the widespread use of intracytoplasmic sperm injection (ICSI), which can overcome instances of infertility previously thought to be intractable. Due to the inherent difficulty of conducting basic research with the limited amounts of human material and governmental bans on basic research of human eggs and pre-implantation embryos, the development of ART hs occurred essentially in the absence of basic research using an animal model system. In essence, this is an example of ART before science. In this grant application, we propose to use the mouse as a model system to study the molecular mechanism and developmental/behavioral consequences of ICSI. The ability of eggs to be activated by parthenogenetic stimuli increases with the time post-hCG administration and correlates with progression of metaphase II-arrested eggs into an interphase-like state. The first Specific Aim will test the hypothesis that, similar to parthenogenetic egg activation, the success of ICSI-induced egg activation increases with time following hCG administration. While ICSI has clearly revolutionized the treatment of male infertility, the molecular basis of how ICSI activates the egg and initiates the program of early development is poorly understood, especially in light of the fact that ICSI bypasses the normal pathway used by the sperm to fertilize the egg. Understanding the molecular basis of egg activation by ICSI will inevitably yield an understanding as to causes for its failure and rationale approaches to enhances its efficacy. The second Specific Aim will test the hypothesis that ICSI recruits signaling pathways that are normally activated during the course of natural fertilization and results in the normal complement of events of egg activation. The rapidly gaining use of ICSI as the method-of-choice to overcome human male infertility has raised a vigorous debate concerning the long-term risk imposed to the resultant offspring. Although it is reassuring at first glance that there is no apparent significant increase in the incidence of gross congenital abnormalities in ICSI-derived offspring, these children are still young and hence more subtle effects that influence behavioral and intellectual development may not yet be apparent. The third Specific Aim will test the hypothesis that mouse ICSI results in subtle developmental and behavior abnormalities in the generated offspring.
辅助生殖技术(ART)的实践要克服

项目成果

期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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RICHARD M SCHULTZ其他文献

RICHARD M SCHULTZ的其他文献

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{{ truncateString('RICHARD M SCHULTZ', 18)}}的其他基金

Gene Expression in the Preimplantation Mouse Embryo
植入前小鼠胚胎中的基因表达
  • 批准号:
    8135897
  • 财政年份:
    2010
  • 资助金额:
    $ 23.37万
  • 项目类别:
Basonuclin and Ribosome Biogenesis in Mouse Oocyte and Embryo
小鼠卵母细胞和胚胎中的基底核蛋白和核糖体生物发生
  • 批准号:
    7760658
  • 财政年份:
    2009
  • 资助金额:
    $ 23.37万
  • 项目类别:
Gene Expression in the Preimplantation Mouse Embryo
植入前小鼠胚胎中的基因表达
  • 批准号:
    7936524
  • 财政年份:
    2009
  • 资助金额:
    $ 23.37万
  • 项目类别:
Basonuclin and Ribosome Biogenesis in Mouse Oocyte and Embryo
小鼠卵母细胞和胚胎中的基底核蛋白和核糖体生物发生
  • 批准号:
    7587729
  • 财政年份:
    2009
  • 资助金额:
    $ 23.37万
  • 项目类别:
Impact of Egg Quality on Gene Expression and Behavior
卵子质量对基因表达和行为的影响
  • 批准号:
    6671981
  • 财政年份:
    2003
  • 资助金额:
    $ 23.37万
  • 项目类别:
Impact of Egg Quality on Gene Expression and Behavior
卵子质量对基因表达和行为的影响
  • 批准号:
    6787309
  • 财政年份:
    2003
  • 资助金额:
    $ 23.37万
  • 项目类别:
Impact of Egg Quality on Gene Expression and Behavior
卵子质量对基因表达和行为的影响
  • 批准号:
    6941214
  • 财政年份:
    2003
  • 资助金额:
    $ 23.37万
  • 项目类别:
Impact of Egg Quality on Gene Expression and Behavior
卵子质量对基因表达和行为的影响
  • 批准号:
    7109360
  • 财政年份:
    2003
  • 资助金额:
    $ 23.37万
  • 项目类别:
Impact of Egg Quality on Gene Expression and Behavior
卵子质量对基因表达和行为的影响
  • 批准号:
    7282057
  • 财政年份:
    2003
  • 资助金额:
    $ 23.37万
  • 项目类别:
Epigenetic Regulation of Imprinting in Mouse Embryo
小鼠胚胎印记的表观遗传调控
  • 批准号:
    6622856
  • 财政年份:
    2002
  • 资助金额:
    $ 23.37万
  • 项目类别:
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