MOLECULAR MECHANISM AND DEVELOPMENT CONSEQUENCES OF ICSI
ICSI 的分子机制和发展后果
基本信息
- 批准号:6151169
- 负责人:
- 金额:$ 23.37万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1999
- 资助国家:美国
- 起止时间:1999-02-01 至 2004-01-31
- 项目状态:已结题
- 来源:
- 关键词:animal developmental psychology assistive reproductive technique biological signal transduction calcium calcium ion chordate locomotion chorionic gonadotropin conditioning egg /ovum embryo /fetus culture embryo /fetus transplantation embryo implantation embryogenesis ethology fertility fertilization gene expression inositol phosphates laboratory mouse microinjections parthenogenesis polymerase chain reaction sperm
项目摘要
The practice of Assisted Reproductive Technology (ART) to overcome
infertility in humans has increased dramatically during the past two
decades and has culminated in the widespread use of intracytoplasmic sperm
injection (ICSI), which can overcome instances of infertility previously
thought to be intractable. Due to the inherent difficulty of conducting
basic research with the limited amounts of human material and governmental
bans on basic research of human eggs and pre-implantation embryos, the
development of ART hs occurred essentially in the absence of basic
research using an animal model system. In essence, this is an example of
ART before science. In this grant application, we propose to use the mouse
as a model system to study the molecular mechanism and
developmental/behavioral consequences of ICSI. The ability of eggs to be
activated by parthenogenetic stimuli increases with the time post-hCG
administration and correlates with progression of metaphase II-arrested
eggs into an interphase-like state. The first Specific Aim will test the
hypothesis that, similar to parthenogenetic egg activation, the success of
ICSI-induced egg activation increases with time following hCG
administration. While ICSI has clearly revolutionized the treatment of
male infertility, the molecular basis of how ICSI activates the egg and
initiates the program of early development is poorly understood,
especially in light of the fact that ICSI bypasses the normal pathway used
by the sperm to fertilize the egg. Understanding the molecular basis of
egg activation by ICSI will inevitably yield an understanding as to causes
for its failure and rationale approaches to enhances its efficacy. The
second Specific Aim will test the hypothesis that ICSI recruits signaling
pathways that are normally activated during the course of natural
fertilization and results in the normal complement of events of egg
activation. The rapidly gaining use of ICSI as the method-of-choice to
overcome human male infertility has raised a vigorous debate concerning
the long-term risk imposed to the resultant offspring. Although it is
reassuring at first glance that there is no apparent significant increase
in the incidence of gross congenital abnormalities in ICSI-derived
offspring, these children are still young and hence more subtle effects
that influence behavioral and intellectual development may not yet be
apparent. The third Specific Aim will test the hypothesis that mouse ICSI
results in subtle developmental and behavior abnormalities in the
generated offspring.
辅助生殖技术(ART)的实践要克服
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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RICHARD M SCHULTZ其他文献
RICHARD M SCHULTZ的其他文献
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{{ truncateString('RICHARD M SCHULTZ', 18)}}的其他基金
Gene Expression in the Preimplantation Mouse Embryo
植入前小鼠胚胎中的基因表达
- 批准号:
8135897 - 财政年份:2010
- 资助金额:
$ 23.37万 - 项目类别:
Basonuclin and Ribosome Biogenesis in Mouse Oocyte and Embryo
小鼠卵母细胞和胚胎中的基底核蛋白和核糖体生物发生
- 批准号:
7760658 - 财政年份:2009
- 资助金额:
$ 23.37万 - 项目类别:
Gene Expression in the Preimplantation Mouse Embryo
植入前小鼠胚胎中的基因表达
- 批准号:
7936524 - 财政年份:2009
- 资助金额:
$ 23.37万 - 项目类别:
Basonuclin and Ribosome Biogenesis in Mouse Oocyte and Embryo
小鼠卵母细胞和胚胎中的基底核蛋白和核糖体生物发生
- 批准号:
7587729 - 财政年份:2009
- 资助金额:
$ 23.37万 - 项目类别:
Impact of Egg Quality on Gene Expression and Behavior
卵子质量对基因表达和行为的影响
- 批准号:
6671981 - 财政年份:2003
- 资助金额:
$ 23.37万 - 项目类别:
Impact of Egg Quality on Gene Expression and Behavior
卵子质量对基因表达和行为的影响
- 批准号:
6787309 - 财政年份:2003
- 资助金额:
$ 23.37万 - 项目类别:
Impact of Egg Quality on Gene Expression and Behavior
卵子质量对基因表达和行为的影响
- 批准号:
6941214 - 财政年份:2003
- 资助金额:
$ 23.37万 - 项目类别:
Impact of Egg Quality on Gene Expression and Behavior
卵子质量对基因表达和行为的影响
- 批准号:
7109360 - 财政年份:2003
- 资助金额:
$ 23.37万 - 项目类别:
Impact of Egg Quality on Gene Expression and Behavior
卵子质量对基因表达和行为的影响
- 批准号:
7282057 - 财政年份:2003
- 资助金额:
$ 23.37万 - 项目类别:
Epigenetic Regulation of Imprinting in Mouse Embryo
小鼠胚胎印记的表观遗传调控
- 批准号:
6622856 - 财政年份:2002
- 资助金额:
$ 23.37万 - 项目类别: