SOCIAL INFLUENCES ON CENTRAL ARGININE VASOTOCIN ACTIONS

社会对中枢精氨酸加压素作用的影响

• 批准号: 6139413
• 负责人: JOHN R GODWIN
• 金额: $ 10.17万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6139413
• 关键词: Osteichthyes; aggression; arginine vasopressin; behavioral /social science research tag; biomarker; ethology; gene expression; hormone receptor; hormone regulation /control mechanism; male; messenger RNA; neural transmission; neuroregulation; protein localization; psychobiology; sex behavior; social behavior; socioenvironment; vasotocin

DESCRIPTION (Applicant's abstract): A long range goal of psychobiology is to understand how internal physiological processes and external environmental factors interact to influence neural function and individual behavior. Improving our understanding of this interaction has strong implications for basic and applied human behavioral biology. The objective of this application is to examine and differentiate social and gonadal hormone influences on the function of the arginine vasotocin/vasopressin (AVT/AVP) system in the brain. The central hypotheses to be tested are: 1) that AVT expression is primarily influenced by the social environment in an animal model system where behavioral sex is determined by dominance interactions rather than the gonads, and 2) that AVT stimulates dominant male-typical sexual and offensive aggressive behavior through central actions in the brain of this teleost fish. The rationale behind this research is that while we know much about how gonadal hormones influence the brain and behavior, much less is known about potential direct social influences on the brain. The AVT system is a logical focus for asking how social influences act on the brain since this family of protein hormones has been strongly linked to the display of sexual behavior in all major groups of vertebrates and to aggression behavior in important mammalian models. The subject of this study, the bluehead wrasse (Thalassoma bifasciatum) undergoes complete behavioral and gonadal female-to-male sex change as an adult animal. This sex change is accompanied by a four- fold increase in AVT-mRNA levels in the brain area which exerts primary control of sexual and aggressive behaviors. The AVT/AVP system is influenced strongly by gonadal steroid hormones in many species. Importantly, however, behavioral sex change can occur even in the absence of gonads in bluehead wrasses and is instead completely dependent on social environment. This species therefore appears to present a unique opportunity to directly examine social influences on this important neural system. To address the central hypotheses above, the PI will pursue three specific aims: 1) Use natural behavioral variation and experimental dissections of social and gonadal influences to examine the control of AVT-mRNA expression, 2) Determine whether AVT can induce sexual and aggressive behavior in this species and 3) Determine sites of AVT action in the wrasse brain by AVT receptor localization and cellular markers of neural activation. Direct influences of social environment on the expression and behavioral actions of AVT would have relevance to humans, where sexual and aggressive behaviors are also under environmental influences.

描述（申请人的摘要）：心理生物学的远程目标 是了解内部生理过程和外部如何 环境因素相互作用以影响神经功能和 个人行为。提高我们对这种互动的理解 对基本和应用的人类行为生物学的强烈影响。这 此应用的目的是检查和区分社会和 性腺激素对精氨酸功能的影响 大脑中的血管素/加压素（AVT/AVP）系统。中央 要测试的假设是：1）AVT表达主要是 在动物模型系统中受社会环境的影响 行为性别是由优势互动决定的，而不是 性腺和2）AVT刺激占主导地位的男性性和 进攻性侵略行为通过大脑中的中心行动 这条硬鱼。这项研究背后的理由是，而我们 了解性腺激素如何影响大脑和行为， 关于潜在的直接社会影响对 脑。 AVT系统是询问社交方式的逻辑重点 影响大脑的作用，因为这种蛋白质激素家族具有 与所有专业的性行为展示密切相关 脊椎动物组和重要哺乳动物的侵略行为 型号。这项研究的主题是蓝头杂志（thalassoma） Bifasciatum）经历完整的行为和性腺女性到男性 作为成年动物的性变化。这种性别改变伴随着四个 大脑区域中AVT-MRNA水平的折叠增加，该水平发挥了原发性 控制性和侵略行为。 AVT/AVP系统是 许多物种受到性腺类固醇激素的强烈影响。 但是，重要的是，即使在 蓝头卷中没有性腺，而是完全 取决于社会环境。因此，这个物种似乎 提供了一个独特的机会，可以直接研究社会影响 这个重要的神经系统。要解决上述中心假设， PI将追求三个具体目标：1）使用自然行为 社会和性腺影响的变化和实验解剖 要检查AVT-MRNA表达的控制，2）确定是否avt 可以在该物种中诱导性和侵略性行为，3） 通过AVT受体确定AVT大脑中AVT动作的位点 神经激活的定位和细胞标记。直接的 社会环境对表达和行为的影响 AVT的行动将与性和性行为相关 侵略行为也受到环境影响。