AMIDE LINKED DNA--HYBRIDZATION BEHAVIOR AND TEMPLATE DIRECTED SYNTHESIS

酰胺连接 DNA——杂交行为和模板定向合成

• 批准号: 6338791
• 负责人: JOHN C LEITZEL
• 金额: $ 15.36万
• 依托单位: CHICAGO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6338791
• 关键词: DNA; amides; biological information processing; chemical stability; high performance liquid chromatography; infrared spectrometry; mass spectrometry; nuclear magnetic resonance spectroscopy; nucleic acid chemical synthesis; nucleic acid hybridization; nucleic acid structure; oligonucleotides

This project investigates the role of DNA backbone modifications in duplex stability and the utility of fully backbone-modified oligomers as model systems for biological information transfer. A series of neutral- backbone DNA analogues are being synthesized and their hybridization behavior systematically studied. These modified oligomers will be further utilized as templates for directing the assembly of complementary substrates. Competition experiments will reveal the relative efficiency of the modified oligomers as templates and substrates relative to native DNA. This research will result in 1) the extension of methodology developed by the PI for assembly of amide-linked DNA analogues by solid-phase synthetic techniques. 2) greater understanding of the factors contributing to the molecular recognition events critical to replication, transcription, and translation, 3) progress in the development of simple in vitro template-directed replicating systems, and 4) the creation of biomedical-related research opportunities for Chicago State University faculty and students in a state of the art laboratory.

本项目研究DNA主链修饰在双链体稳定性中的作用，以及完全主链修饰的寡聚体作为生物信息传递模型系统的实用性。一系列中性骨架DNA类似物的合成和杂交行为的系统研究正在进行中.这些修饰的低聚物将进一步用作模板，用于指导互补底物的组装。竞争实验将揭示修饰的寡聚物作为模板和底物相对于天然DNA的相对效率。本研究将导致1）PI开发的用于通过固相合成技术组装酰胺连接的DNA类似物的方法的扩展。2)更好地了解分子识别事件的因素，这些事件对复制、转录和翻译至关重要，3）在简单的体外模板指导复制系统的开发方面取得进展，以及4）在最先进的实验室中为芝加哥州立大学的教师和学生创造生物医学相关的研究机会。