AMIDE LINKED DNA--HYBRIDZATION BEHAVIOR AND TEMPLATE DIRECTED SYNTHESIS

本项目研究DNA骨架修饰在双链稳定性中的作用，以及完全骨架修饰的寡聚体作为生物信息传输模型系统的用途。人们正在合成一系列中性骨架DNA类似物，并对它们的杂交行为进行了系统的研究。这些修饰的低聚物将进一步用作指导互补底物组装的模板。竞争实验将揭示修饰低聚物作为模板和底物相对于天然DNA的相对效率。这项研究将导致1)PI开发的用于用固相合成技术组装酰胺连接的DNA类似物的方法的扩展。2)更好地了解对复制、转录和翻译至关重要的分子识别事件的因素，3)简单的体外模板导向复制系统的开发进展，以及4)在最先进的实验室为芝加哥州立大学的教职员工和学生创造与生物医学相关的研究机会。

This project investigates the role of DNA backbone modifications in duplex stability and the utility of fully backbone-modified oligomers as model systems for biological information transfer. A series of neutral- backbone DNA analogues are being synthesized and their hybridization behavior systematically studied. These modified oligomers will be further utilized as templates for directing the assembly of complementary substrates. Competition experiments will reveal the relative efficiency of the modified oligomers as templates and substrates relative to native DNA. This research will result in 1) the extension of methodology developed by the PI for assembly of amide-linked DNA analogues by solid-phase synthetic techniques. 2) greater understanding of the factors contributing to the molecular recognition events critical to replication, transcription, and translation, 3) progress in the development of simple in vitro template-directed replicating systems, and 4) the creation of biomedical-related research opportunities for Chicago State University faculty and students in a state of the art laboratory.