BIOFUNCTIONAL MATERIALS CONTAINING PROTEIN MIMETICS

含有蛋白质模拟物的生物功能材料

• 批准号: 6024042
• 负责人: WEIYUAN J KAO
• 金额: $ 21.01万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6024042
• 关键词: biological signal transduction; biomaterial development /preparation; cell adhesion; clinical research; complement; cytokine receptors; enzyme linked immunosorbent assay; fibronectins; growth factor; human subject; interleukin 1; laboratory rat; leukocyte activation /transformation; ligands; macrophage; oligopeptides; protein structure function; receptor binding

Blood monocyte-derived macrophages (a class of phagocytic leukocytes) play a critical role in directing inflammation and foreign body reaction to biomaterials. However, the processes leading to macrophage adhesion and activation on materials are complex and not yet fully understood. Hence, our interest in macrophage interaction with material macrophage adhesion and activation. Second, we would like to exploit that knowledge in the design of materials and fundamental understanding of the interplay between macrophage-active proteins and receptors on the cell membranes must be obtained. Oligopeptides will be designed based on the known structural structure of modulating inflammation. These protein mimetic peptides will be utilized to probe the molecular mechanisms required for ligand-receptor recognition and the induction of cellular functions. In term, the bioactive peptides production. Heterofunctionalized polymeric materials will be developed and optimized to enhanced the physicochemical properties and bioavailability of macrophage-active oligopeptides in vitro and in vivo. This research plan offers a systematic method in the study of protein-receptor interaction and the development of material constructs designed to modulate the release of selected cytokines and growth factors produced by endogenous inflammatory cells. The controlled release of these bioactive factors may have therapeutic values in the fundamental processes of inflammation, biocompatibility, and tissue healing.

血液单核细胞衍生的巨噬细胞（一类吞噬性白细胞）在引导炎症和对生物材料的异物反应中起关键作用。然而，导致巨噬细胞粘附和激活材料的过程是复杂的，尚未完全理解。因此，我们对巨噬细胞与材料巨噬细胞粘附和活化的相互作用感兴趣。第二，我们想利用材料设计中的知识，并且必须获得对巨噬细胞活性蛋白和细胞膜上受体之间相互作用的基本理解。寡肽将基于已知的调节炎症的结构来设计。这些蛋白质模拟肽将用于探测配体-受体识别和诱导细胞功能所需的分子机制。在术语中，生物活性肽的生产。杂功能化聚合物材料将被开发和优化，以提高巨噬细胞活性寡肽的体外和体内的理化性质和生物利用度。该研究计划提供了一种系统的方法，用于研究蛋白质-受体相互作用和开发旨在调节内源性炎症细胞产生的选定细胞因子和生长因子释放的材料结构。这些生物活性因子的受控释放可能在炎症、生物相容性和组织愈合的基本过程中具有治疗价值。