AXON--SCHWANN INTERACTIONS IN VITRO

描述：所描述的工作的长期目标是了解如何 细胞外信号协调雪旺细胞表型的变化。 待检验的主要假设是Ras/MAP激酶途径作用于 通过转录因子AP-2和C/EBP增加髓鞘P0表达 而触发这一通路的细胞外信号是层粘连蛋白， 基底层成分。 这项提案的目的是调查 是否MAP激酶和cAMP激活髓鞘基因发生通过这些 两个转录因子，并证明层粘连蛋白诱导的 髓磷脂基因利用ras途径。 理解机理 由此调节许旺细胞表型可以允许实验性的 操纵这种细胞可能在几个医学领域有帮助 例如设计脱髓鞘疾病和移植的疗法 用于脊髓再生

DESCRIPTION: The long-term goal of the work described is to understand how extracellular signals orchestrate changes in the Schwann cell phenotype. The main hypothesis to be tested is that the Ras/MAP kinase pathway acting through transcription factors AP-2 and C/EBP increases myelin P0 expression and the extracellular signal that triggers this pathway is laminin, a component of basal lamina. The aims of this proposal are to investigate whether MAP kinase and cAMP activation of myelin genes occur through these two transcription factors and to demonstrate that laminin induction of myelin genes utilizes the ras pathway. An understanding of the mechanism whereby the Schwann cell phenotype is regulated can allow for experimental manipulation of this cell which might be helpful in several medical areas such as designing therapies for demyelinating diseases and transplantation for spinal cord regeneration.