NEUROBIOLOGY OF HYPOTHALAMIC PAIN MODULATION

疼痛是护士非常关注的一个重大健康问题。最痛苦的是- 相关护理研究涉及疼痛测量和 干预，但要优化这样的研究和对如何 身体对到来的疼痛刺激进行调整是必要的。建议数 研究探讨外侧下丘脑在内源性脑损伤中的作用 在大鼠模型中对疼痛或止痛的调制。因为 外侧下丘脑与脊髓的直接联系很少 脊髓背角，疼痛调制的关键部位，很可能是 外侧下丘脑通过其他脑引起镇痛 结构。使用轨迹追踪和免疫细胞化学相结合， 外侧核之间联系的神经化学特性 减轻疼痛刺激的下丘脑和四个脑干核团 将会被研究。这四个核团是中脑导水管周围灰质， 桥脑背外侧被盖、中脑内侧延髓和延髓 桥脑脚被盖。确定的职能角色 下丘脑外侧区参与痛觉调制，多种受体激动剂 拮抗剂将被微量注射到这些大脑结构中 诱导并阻断一种止痛效果。止痛将会是 用甩尾法和足部撤除法进行测量， 标准的止痛测试。数据将使用以下工具进行分析 描述性统计和双向重复测量方差分析。这个 这项研究的结果将提供更好的理解 内源性疼痛调节系统，可导致改善 药理学和非药理学人类疼痛管理 学习。

Pain is a major health issue of great concern to nurses. Most pain- related nursing research is concerned with pain measurement and intervention, but to optimize such research and understanding of how the body modifies incoming pain stimuli is necessary. The proposed study investigates the role of the lateral hypothalamus in endogenous modulation of pain, or analgesia, in a rat model. Because the lateral hypothalamus makes few direct connections with the spinal cord dorsal horn, a key site for pain modulation, it is likely that the lateral hypothalamus evokes analgesia through other brain structures. Using tract-tracing combined with immunocytochemistry, the neurochemical identity of connections between the lateral hypothalamus and four brainstem nuclei that attenuate painful stimuli will be studied. These four nuclei are the periaqueductal grey, the dorsolateral pontine tegmentum, the benntromedial medulla and the pedunculopontine tegmentum. To determine a functional role for the lateral hypothalamus in pain modulation, various receptor agonists and antagonists will be micro injected into these brain structures to induce, and then block, an analgesic effect. Analgesia will be measured using the tail flick and foot withdrawal latencides, standard analgesiometic tests. Data will be analyzed using descriptive statistics and two-way repeated measures ANOVA. The findings of this study will provide better understanding of endogenous pain modulatory systems which can lead to improved pharmacological and non-pharmacological human pain management studies.