NEUROBIOLOGY OF HYPOTHALAMIC PAIN MODULATION

下丘脑疼痛调节的神经生物学

• 批准号: 6685324
• 负责人: JANEAN E HOLDEN
• 金额: $ 23.09万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2005-04-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6685324
• 关键词: analgesia; central neural pathway /tract; hypothalamus; immunocytochemistry; laboratory rat; microinjections; neural information processing; neuroanatomy; neurobiology; neurochemistry; neurotensin; pain; periaqueductal gray matter; stimulus /response; substance P; tegmentum

Pain is a major health issue of great concern to nurses. Most pain- related nursing research is concerned with pain measurement and intervention, but to optimize such research and understanding of how the body modifies incoming pain stimuli is necessary. The proposed study investigates the role of the lateral hypothalamus in endogenous modulation of pain, or analgesia, in a rat model. Because the lateral hypothalamus makes few direct connections with the spinal cord dorsal horn, a key site for pain modulation, it is likely that the lateral hypothalamus evokes analgesia through other brain structures. Using tract-tracing combined with immunocytochemistry, the neurochemical identity of connections between the lateral hypothalamus and four brainstem nuclei that attenuate painful stimuli will be studied. These four nuclei are the periaqueductal grey, the dorsolateral pontine tegmentum, the benntromedial medulla and the pedunculopontine tegmentum. To determine a functional role for the lateral hypothalamus in pain modulation, various receptor agonists and antagonists will be micro injected into these brain structures to induce, and then block, an analgesic effect. Analgesia will be measured using the tail flick and foot withdrawal latencides, standard analgesiometic tests. Data will be analyzed using descriptive statistics and two-way repeated measures ANOVA. The findings of this study will provide better understanding of endogenous pain modulatory systems which can lead to improved pharmacological and non-pharmacological human pain management studies.

疼痛是护理人员非常关注的一个主要健康问题。 最痛苦的- 相关的护理研究关注疼痛测量， 干预，但要优化这种研究和了解如何 身体调整传入的疼痛刺激是必要的。 拟议 一项研究探讨了外侧下丘脑在内源性 调节大鼠模型中的疼痛或镇痛。 因为 外侧下丘脑与脊髓的直接联系很少 脊髓背角是疼痛调节的关键部位， 外侧下丘脑通过其他脑引起镇痛 结构.采用示踪结合免疫细胞化学的方法， 侧支与侧支之间连接的神经化学特性 下丘脑和四个脑干核团，它们减弱疼痛刺激 将被研究。 这四个核团分别是中脑导水管周围灰质， 背外侧脑桥被盖、延髓内侧和 脚桥被盖 要确定 外侧下丘脑在疼痛调节中的作用，各种受体激动剂 拮抗剂将被微量注射到这些大脑结构中， 诱导然后阻断镇痛作用。 镇痛将是 使用甩尾和缩足潜伏剂测量， 标准光镜检查。 将使用以下方法分析数据： 描述性统计和双向重复测量方差分析。 的 这项研究的结果将有助于更好地了解 内源性疼痛调节系统，其可导致改善的 药理学和非药理学人类疼痛管理 问题研究