ADVANCED DEVELOPMENT/TESTING OF A 384-CAPILLARY SEQUENCE

描述：（申请人的摘要）高通量和自动化DNA 需要降低测序成本的测序仪器。 多毛细管电泳已被证明能够满足这一需求。 这项技术现在已经很成熟，并已被证明是一个重要的 比以前的平板凝胶技术更先进。然而，很明显， 进一步的开发可以显著提高可靠性，降低 每个样品的仪器成本，以及增加的灵敏度， 更小的测序样品和伴随的低得多的测序成本。 SpectruMeetings Corporation的多毛细管技术使用“柱上” 检测，其具有优于在其它环境中使用的检测方法的若干优点。 毛细管阵列测序仪。一个优点是它允许同时 检测384个毛细管，从而将96个毛细管的仪器升级为 384毛细管仪器可以通过仅仅升级光学 检测系统包括CCD摄像机、摄像机透镜和激光器， 而不会在性能上有任何妥协。这些升级已经过测试， 经验证性能良好。这个384毛细管仪器将显着 增加毛细管阵列电泳通量。 一旦384毛细管测序仪原型组装完成，它将被送往 测序生产设施，以优化其兼容性，导致全面 整合和利用。吞吐量的主要增长与 进一步的技术改进以产生超过1200个碱基的读取长度 将允许显著降低每个样品和每个测序碱基的成本。

DESCRIPTION: (Applicant's Abstract) High-throughput and automated DNA sequencing instrumentation that reduces the cost of sequencing is needed. Multiple-capillary electrophoresis has proven capable of meeting this need. This technology is now well established and has proven to be a significant advance over the previous slab gel technology. However, it is apparent that further development can lead to significantly increased reliability, lower instrument costs per sample, and increased sensitivity that would allow much smaller sequencing samples and concomitantly much lower sequencing costs. SpectruMedix Corporation's multiple-capillary technology uses "on-column" detection, which has several advantages over detection methods used in other capillary array sequencers. One advantage is that it allows simultaneous detection of 384 capillaries, so that upgrading the 96-capillary instrument to a 384-capillary instrument can be accomplished by merely upgrading the optical detection system including the CCD camera, the camera lens, and the laser, without any compromises in performance. These upgrades have been tested and verified to perform well. This 384-capillary instrument will dramatically increase capillary array electrophoresis throughput. Once the 384-capillary sequencer prototype is assembled, it will be sent to a sequencing production facility to optimize its compatibility leading to full integration and utilization. The major increase in throughput combined with further technology improvements to yield in excess of 1200 base read-length will allow dramatic cost reduction per sample and per sequenced base.