ADVANCED DEVELOPMENT/TESTING OF A 384-CAPILLARY SEQUENCE

384 毛细管序列的高级开发/测试

• 批准号: 6312500
• 负责人: Tom E. Kane
• 金额: $ 71.28万
• 依托单位: SPECTRUMEDIX CORPORATION
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6312500
• 关键词: biomedical equipment development; biotechnology; capillary electrophoresis; high throughput technology; nucleic acid sequence

DESCRIPTION: (Applicant's Abstract) High-throughput and automated DNA sequencing instrumentation that reduces the cost of sequencing is needed. Multiple-capillary electrophoresis has proven capable of meeting this need. This technology is now well established and has proven to be a significant advance over the previous slab gel technology. However, it is apparent that further development can lead to significantly increased reliability, lower instrument costs per sample, and increased sensitivity that would allow much smaller sequencing samples and concomitantly much lower sequencing costs. SpectruMedix Corporation's multiple-capillary technology uses "on-column" detection, which has several advantages over detection methods used in other capillary array sequencers. One advantage is that it allows simultaneous detection of 384 capillaries, so that upgrading the 96-capillary instrument to a 384-capillary instrument can be accomplished by merely upgrading the optical detection system including the CCD camera, the camera lens, and the laser, without any compromises in performance. These upgrades have been tested and verified to perform well. This 384-capillary instrument will dramatically increase capillary array electrophoresis throughput. Once the 384-capillary sequencer prototype is assembled, it will be sent to a sequencing production facility to optimize its compatibility leading to full integration and utilization. The major increase in throughput combined with further technology improvements to yield in excess of 1200 base read-length will allow dramatic cost reduction per sample and per sequenced base.

描述：(申请人摘要)高通量和自动化的DNA 需要能够降低测序成本的测序仪器。 多毛细管电泳法已被证明能够满足这一需求。 这项技术现在已经很成熟，并已被证明是一项重要的 比以前的板条凝胶技术有了进步。然而，很明显， 进一步的开发可以显著提高可靠性，降低 每个样本的仪器成本，以及更高的灵敏度，这将允许 更小的测序样本和随之而来的更低的测序成本。 SpectruMedex公司的多毛细管技术采用“柱上”技术 检测，这与其他使用的检测方法相比有几个优点 毛细管阵列测序仪。一个优点是它允许同时 检测384根毛细管，从而将96根毛细管仪器升级到 一台384毛细管的仪器只需升级光学仪器即可完成 检测系统包括CCD摄像机、摄像机镜头和激光器， 在性能上不会有任何妥协。这些升级已经过测试， 已验证运行良好。这台384毛细管仪器将戏剧性地 增加毛细管阵列电泳量。 一旦384毛细管测序仪原型组装完成，它将被送往一个 测序生产设施，优化其兼容性，导致完全 整合利用。吞吐量的主要增长与 进一步的技术改进，使产量超过1200个基本读取长度 将大幅降低每个样品和每个测序碱基的成本。