3 D HOLOGRAPHIC VIDEO RECORDING IN BIOLOGICAL TISSUE

生物组织中的 3D 全息视频记录

• 批准号: 6085481
• 负责人: DAVID D NOLTE
• 金额: $ 9.81万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6085481
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; burns; catheterization; charge coupled device camera; endoscopy; holography; skin neoplasms; tissue /cell culture; video microscopy; video recording system

DESCRIPTION (Adapted from the applicant's abstract): The applicants propose to develop an optical biomedical imaging technique that records real-time video biological tissue, without computed tomography, using compact light sources. This technique uses a newly developed adaptive holographic film that performs as a coherence filter on a video camera, making it possible to image up to 15 mean-free-scattering paths into strongly scattering living tissue near- infrared light (up to 100 um deep into tissue while maintaining 10 um spatially high resolution). The capabilities of the technique will be benchmarked by imaging the three-dimensional structure multicellular spheroids composed of normal and neoplastic cells (tumor spheroids). The spheroids are balls of cells that may be easily cultured in vitro and used to simulate the optical properties of various tissues and nodular tumors. If successful, the proposed research has many biomedical applications. For instance, this technology may be used for dermatological lesion scanning (skin cancer), burn assessment, investigation of internal surfaces through endoscopes or catheters using imagin bundles, and intra-operative procedures giving a physician immediate video images of tissue beneath the surgical surface. Because of the immediate high-speed nature of direct imaging, this technology will be particularly attractive for fast dynamic studies and making movies of processes inside tissue such as portions of the cardiovascular system requiring high time resolution, or for metabolic imaging of tumors and the rapid uptake of tagged optical markers. This optical tissue imaging technology will be unique in its combination of high time resolution with high spatial resolution, and therefore represents a unique biomedical imaging technology with significant potential for down-stream development for cancer imaging.

描述（改编自申请人摘要）：申请人建议 开发一种记录实时视频的光学生物医学成像技术 生物组织，没有计算机断层扫描，使用紧凑的光源。 这项技术使用了一种新开发的自适应全息薄膜， 作为摄像机上的相干滤波器，使其能够成像高达15 平均自由散射路径进入强散射活体组织附近， 红外光（深入组织达100 um，同时保持10 um 空间高分辨率）。 该技术的能力将是 通过对三维结构的多细胞球体进行成像 由正常细胞和肿瘤细胞（肿瘤球体）组成。 球状体 细胞球，可以很容易地在体外培养，并用于模拟 各种组织和结节性肿瘤的光学特性。 如果成功，则 拟议的研究有许多生物医学应用。 比如说这个 该技术可用于皮肤病损扫描（皮肤癌）、烧伤 通过内窥镜或导管对内表面进行评估和研究 使用成像束和术中程序， 手术表面下组织的即时视频图像。 因为 由于直接成像的即时高速性，这项技术将成为 对于快速动态研究和制作电影特别有吸引力， 组织内的过程，例如心血管系统的部分 需要高时间分辨率，或者用于肿瘤的代谢成像， 标记的光学标记物的快速摄取。 这种光学组织成像 这项技术将是独一无二，它将高时间分辨率与高 空间分辨率，因此代表了独特的生物医学成像 对癌症下游发展具有重大潜力技术 显像