FUNCTIONAL GENOMICS AND PROTEOMICS BIOTECHNOLOGY CENTER

描述（申请摘要） 最复杂的生物功能涉及同时表达 数百甚至数千个基因和蛋白质。最新技术 的进展和大型综合序列数据库的建立， 各种基因组测序项目的成果 可以分析全局 mRNA 和蛋白质表达模式。新的 技术进步将促进基因和蛋白质表达的研究 概况包括高密度核酸微阵列和质量 基于光谱仪的蛋白质组学。高昂的成本和缺乏专业技术 限制了许多研究人员对这些技术的使用。因此，合理的 使基因组学和蛋白质组学技术更容易获得的解决方案是 建立多用户生物技术中心。我们建议设立一个 辛辛那提大学医学中心 NIDDK 生物技术中心 （医学院和儿童医院）。有 51 个 NIDDK 资助 加州大学医学中心的研究人员。此外，拟议的生物技术 该中心将成为 NIDDK 资助的位于附近三个研究人员的资源 机构：俄亥俄州立大学（n=17）；肯塔基大学 (n=8)；和 路易斯维尔大学 (n=8)。我们已经开始开发这两种 mRNA （基因组学）和蛋白质（蛋白质组学）分析设施，配备人员 与训练有素的人员。机构资金已用于 采购大部分主要设备。生物技术的资助将允许 我们扩大我们的能力，以便可以提供四项优先服务 NIDDK研究人员：1）构建高质量的定制消减cDNA 库，用于制作微阵列模板，2) 机器人打印 将高密度 cDNA 微阵列置于载玻片上，3) 二维 (2-D) 使用最先进的质谱法进行蛋白质凝胶分析和蛋白质组学， 4）生物信息学支持。我们的高通量能力 设施可以为大量人员提供基因组学和蛋白质组学支持 NIDDK 资助的调查人员。此外，我们还将提供使用培训 向研究生介绍全球基因和蛋白质表达技术， 博士后研究员和教员。

DESCRIPTION (Abstract of the application) The most complex biological functions involve the simultaneous expression of hundreds, or perhaps thousands of genes and proteins. Recent technological advances and the establishment of large comprehensive sequence databases, which have resulted from the various genome-sequencing projects, have made it possible to analyze global mRNA and protein expression patterns. The new technological advances will facilitate the study of gene and protein expression profiles include high-density nucleic acid microarrays and mass spectrometer-based proteomics. High costs and lack of technical expertise have limited the use of these technologies by many investigators. Thus, a reasonable solution for making genomics and proteomics technologies more accessible is the establishment of multi-user Biotechnology Centers. We propose to establish a NIDDK Biotechnology Center at the University of Cincinnati Medical Center (College of Medicine and Children's Hospital). There are 51 NIDDK funded investigators at the UC Medical Center. In addition, the proposed Biotechnology Center will be a resource for NIDDK funded investigators at three nearby institutions: Ohio State University (n=17); University of Kentucky (n=8); and University of Louisville (n=8). We have already begun developing both mRNA (genomics) and protein (proteomics) profiling facilities, which are staffed with highly trained individuals. Institutional funding has been used to purchase most of the major equipment. Funding of the Biotechnology will allow us to expand our capabilities so that four priority services can be provided to NIDDK investigators: 1) the construction of high-quality custom subtracted cDNA libraries, which are used to make microarray templates, 2) the robotic printing of high-density cDNA microarrays onto glass slides, 3) 2-dimensional (2-D) protein gel analysis and proteomics using state-of-the-art mass spectrometry, and 4) bioinformatics support. The high-throughput capabilities of our facilities can provide both genomics and proteomics support to a large number of NIDDK-funded investigators. In addition, we will provide training in the use of global gene and protein expression technologies to graduate students, post-doctoral fellows, and faculty members.