GENOME STABILITY IN RAD MUTANTS

同源重组是一种重要的DNA生物学反应 辐射和化学物质的损害。 然而，重组必须 控制，因为有害的基因组重排结果， 重复序列之间的重组。在几个观察 真核系统已经建立了序列之间的重组 长度在250 bp以下受到严格控制。 这对于 维持基因组的稳定性，因为大多数重复的 序列很短。在这个实验室里， (RAD)基因已经被证明控制短的之间的重组， 酵母S.酿酒酵母，这种控制是不同的， 控制较长序列之间的重组。 进一步 这种选择性控制的调查是建议的目标， 研究计划。 新的缺失和易位分析将是 用于确定额外的RAD基因在控制中的作用 短重复序列重组（SRR） 这些基因在双- 链断裂（DSB）加工、异源双链体形成和连接 SRR过程中的分子形成将用一种新的DSB诱导 基因置换测定。这些基因和身体研究将 提供了独特的见解，如何保持基因组的完整性， DNA损伤。

Homologous recombination is an important biological response to DNA damage by radiation and chemicals. However, recombination must be controlled because deleterious genome rearrangements results from recombination between repeated sequences. Observations in several eukaryotic systems have established the recombination between sequences under 250 bp in length is tightly controlled. This is essential for the maintenance of genome stability because the majority of repeated sequences are short. In this laboratory, several radiation sensitivity (RAD) genes have been shown to control recombination between short sequences in the yeast S. cerevisiae, and that this control is distinct from the control of recombination between longer sequences. The further investigation of this selective control is the goal of the proposed research program. Novel deletion and translocation assays will be employed to determine the role of additional RAD genes in the control of short-repeat recombination (SRR). The role of these genes in double- strand break (DSB) processing, heteroduplex formation, and joint molecular formation during SRR will be analyzed with a novel DSB induced gene replacement assay. Together these genetic and physical studies will provide unique insight into how genomic integrity is maintained in the wake of DNA damage.