2- AND 3-D IMAGING OF METABOLIC ACTIVITION OF BETA CELLS

快速冷冻胰腺的高分辨率（50亩）二维和三维成像显示，在可行性研究中，NADH(P)H和黄蛋白（Fp）减少和血红蛋白浓度增加的独特局部信号响应葡萄糖挑战。这一结果鼓励对葡萄糖和亮氨酸滴定反应的图像进行比较，以区分线粒体和细胞质NADH(P)H的反应。氧化还原变化的时间分辨成像提供了进一步的区别；预计细胞质反应将先于线粒体（Fp）反应，这些时间分离将通过葡萄糖/亮氨酸比较来证实。β细胞反应的鉴定将通过组织切片的组织病理学来证实，这些组织切片是从最后一次光学扫描的组织块上处理和切片的。预计这些快速冷冻成像研究将为未来基于方法2（飞行荧光）和方法3（近红外经腹成像）的体内β细胞功能的微创光学评估提供理论依据。

High resolution (50 mu)2- and 3-dimensional imaging of snap frozen pancreas shows in feasibility studies distinctive localized signals from NADH(P)H and flavoprotein (Fp) reduction and hemoglobin concentration increases in response to glucose challenges. This result encourages comparison of images in response to titrations with glucose and leucine in order to differentiate the response of mitochondrial and cytosolic NADH(P)H. A further distinction is afforded by time-resolved imaging of redox changes; it is expected that cytosolic responses will precede mitochondrial (Fp) responses and these time separations will be confirmed by glucose/leucine comparisons. Identification of beta cell responses will be confirmed by histopathology of tissue sections processed and microtomed from the tissue block which the last optical scan is made. It is expected that these snap frozen imaging studies will afford a rationale for future minimally invasive optical evaluation of beta cell function in vivo base upon Method 2, Flying Fluorescence and Methods 3, NIR Transabdominal Imaging.