BREAST CANCER HYPOXIA ASSESSED BY NITRIC OXIDE

描述：(申请人描述) 我们在这里的目的是为翻译研究奠定基础 近红外光学法测定人体低氧水平的效果 用近红外光谱分析和成像技术对乳腺癌进行检测。该测试需要一个 PO2提供的乳腺肿瘤缺氧的金标准 确定仪器、专业知识和技术在哪里 在莱比锡大学提供，而近红外的专业知识和技术 宾夕法尼亚大学提供光谱学和成像技术 为翻译研究提供了机会。我们的意图是 动员这两个地点的活动，以便初步测试50 乳腺癌可以用来验证光谱学和成像 乳腺肿瘤乏氧的测定值与金的测定值相当 氧分压的测定标准和 肿瘤定位与其他检查方法尤其一致。 超声波，乳房X光检查和活组织检查。因此，这些研究需要验证 这两个小组可以合作；费城小组提供 仪器和熟练使用仪器的人员，同时 莱比锡小组不仅将提供PO2测定方面的专业知识， 超声波、活组织检查、磁共振成像，而且还能提供 大量高危乳腺癌患者愿意 接受完整的乳腺癌检查方案。这是我们的意图 提供两种类型的近红外信息：i)定位的和定量的 高血压病患者血红蛋白去饱和度和血液含量的光谱分析 与PO2测定发生的乳腺癌区域相同。 2)提供快速有效成像的近红外成像系统 血红蛋白脱氧率和血药浓度与正常范围的比较 乳房组织。PO2测定和近红外血红蛋白光谱分析 和成像目前为模型中的校准提供了基础， 以前从未在人类乳腺癌中进行过比较研究。如果 达到了我们预期的论证可行性的目标，然后应用 通过常规RO1渠道提供支持似乎是合理的。

DESCRIPTION: (Applicant's Description) It is our intent here to lay the groundwork for a translational study of the efficacy of NIR optical method in deternining the level of hypoxia in human breast cancer by NIR optical spectroscopy and imaging. The test requires a gold standard of breast tumor hypoxia which is to be provided by pO2 deterninations where the instrumentation, expertise and technology is available at the Leipzig University while the expertise and technology for NIR spectroscopy and imaging is available at the University of Pennsylvania affording an opportunity for translational research. It is our intent to mobilize activities at these two locations in order that a initial test of 50 breast cancers can be made to validate that the spectroscopy and imaging of breast tumor hypoxia gives values comparable to those obtained with the gold standard of pO2 determination and that the tumor localization is consistent with other modalities particularly ultrasound, mammography and biopsy. Thus these studies require validation that these two groups can work together; the Philadelphia group providing instrumentation and personnel skilled in use of the instrumentation, whilst the Leipzig group will provide not only expertise in pO2 determination, ultrasound, biopsy, marnnography, but also the capability of making available a large population of high risk breast cancer patients who are willing to accept the complete protocol for breast cancer examinations. It is our intent to provide two types of NIR information: I) localized and quantitative spectroscopy of the desaturation of hemoglobin and the blood content in the same breast canceri region as that in which the pO2 deternination takes place. 2) A NIR imaging system which gives rapid and effective imaging of the deoxygenation of hemoglobin and blood concentration relative to nearby normal breast tissue. While the pO2 determinations and NIR hemoglobin spectroscopy and imaging currently afford the basis for calibration in models, the comparative study has never been done before in human breast cancers. If ourintended goals of demonstrating feasibility are achieved, then application for support through regular RO1channels would appear to be justified.