BREAST CANCER HYPOXIA ASSESSED BY NITRIC OXIDE

通过一氧化氮评估乳腺癌缺氧

• 批准号: 6514636
• 负责人: BRITTON CHANCE
• 金额: $ 10.34万
• 依托单位: MEDICAL DIAGNOSTIC RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-16 至 2004-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6514636
• 关键词: breast neoplasms; clinical research; hemoglobin; human subject; hypoxia; infrared spectrometry; metastasis; nitric oxide; polarography

DESCRIPTION: (Applicant's Description) It is our intent here to lay the groundwork for a translational study of the efficacy of NIR optical method in deternining the level of hypoxia in human breast cancer by NIR optical spectroscopy and imaging. The test requires a gold standard of breast tumor hypoxia which is to be provided by pO2 deterninations where the instrumentation, expertise and technology is available at the Leipzig University while the expertise and technology for NIR spectroscopy and imaging is available at the University of Pennsylvania affording an opportunity for translational research. It is our intent to mobilize activities at these two locations in order that a initial test of 50 breast cancers can be made to validate that the spectroscopy and imaging of breast tumor hypoxia gives values comparable to those obtained with the gold standard of pO2 determination and that the tumor localization is consistent with other modalities particularly ultrasound, mammography and biopsy. Thus these studies require validation that these two groups can work together; the Philadelphia group providing instrumentation and personnel skilled in use of the instrumentation, whilst the Leipzig group will provide not only expertise in pO2 determination, ultrasound, biopsy, marnnography, but also the capability of making available a large population of high risk breast cancer patients who are willing to accept the complete protocol for breast cancer examinations. It is our intent to provide two types of NIR information: I) localized and quantitative spectroscopy of the desaturation of hemoglobin and the blood content in the same breast canceri region as that in which the pO2 deternination takes place. 2) A NIR imaging system which gives rapid and effective imaging of the deoxygenation of hemoglobin and blood concentration relative to nearby normal breast tissue. While the pO2 determinations and NIR hemoglobin spectroscopy and imaging currently afford the basis for calibration in models, the comparative study has never been done before in human breast cancers. If ourintended goals of demonstrating feasibility are achieved, then application for support through regular RO1channels would appear to be justified.

描述：（申请人的描述） 我们在这里的目的是为翻译研究奠定基础， 近红外光学法测定人体缺氧水平的有效性 乳腺癌的近红外光谱和成像。测试需要一个 pO 2提供的乳腺肿瘤缺氧的金标准 仪器、专业知识和技术 而近红外光谱的专业知识和技术， 光谱学和成像技术在宾夕法尼亚大学 为转化研究提供了机会。我们的目的是 在这两个地点动员活动，以便在50个 乳腺癌可以用来验证 乳腺肿瘤缺氧给出的值与用金获得的值相当 pO 2测定标准， 肿瘤定位与其他方式一致， 超声波、乳房X光检查和活检。因此，这些研究需要验证， 这两个小组可以一起工作;费城小组提供 仪器和熟练使用仪器的人员，同时 莱比锡小组不仅将提供pO 2测定方面专门知识， 超声波，活检，mannography，但也有能力使可用 大量的高危乳腺癌患者愿意接受 接受完整的乳腺癌检查方案。我们的目的是 提供两种类型的NIR信息：I）局部和定量 血红蛋白的去饱和度和血液含量的光谱分析， 与pO 2测定发生的乳腺癌区域相同。 2)近红外成像系统，可快速有效地对物体进行成像 血红蛋白脱氧和相对于附近正常值的血液浓度 乳房组织虽然pO 2测定和近红外血红蛋白光谱 和成像目前提供了校准模型的基础， 以前从未在人类乳腺癌中进行过比较研究。如果 达到了论证可行性的预期目标， 通过常规RO 1渠道寻求支持似乎是合理的。