FLUORESCENT MARKERS FOR BREAST CANCER SURGERY

描述：（申请人的描述） 本项目的目的是合成六种荧光钴胺素 衍生物并选择最佳化合物用于改善肿瘤 乳腺癌前哨淋巴结活检过程中的标记。我们的 迄今为止的工作表明，所有测试的癌症和白血病细胞系都具有 对钴胺素（维生素 B12）的需求异常高。荧光灯 钴胺素衍生物应该是一个很好的视觉标记，专门针对 癌细胞，从而有助于视觉识别转移性肿瘤 淋巴管和淋巴结疾病。荧光钴胺素缀合物也可以 改善肿瘤微切缘的清晰度并有助于手术 切除癌组织，同时保留健康组织。作为一个 转移性乳腺疾病的术中标志物，它可能会降低 目前前哨淋巴结活检程序的假阴性率 并通过以下方式为患者提供更好的生活质量： 保存健康的乳腺组织和健康的轴向淋巴结。这个 提案将为荧光素-钴胺素的初步评估提供资金 我们现在手头有衍生品，并为合成和评估提供资金 几种第二代荧光钴胺素结合物 比我们现有的化合物更明亮、更耐光。荧光 钴胺素结合物将在已切除的原发性人类肿瘤中进行测试 来自接受乳腺癌手术的患者。

DESCRIPTION: (Applicant's Description) The purpose of this project is to synthesize six fluorescent cobalamin derivatives and select the optimal compound for use as an improved tumor marker in the sentinel lymph node biopsy procedure for breast cancer . Our work to date has shown that all cancer and leukemia cell lines tested have an unusually high requirement for cobalamin (Vitamin B12). A fluorescent derivative of cobalamin should be a good visual marker that is specific for cancer cells and thereby aid in the visual identification of metastatic disease in lymph ducts and nodes. A fluorescent cobalamin conjugate may also improve the definition of tumor micro margins and aid in the surgical excision of cancerous tissue while sparing healthy tissue. As an intraoperative marker for metastatic breast disease, it may decrease the false-negative rate of the sentinel node biopsy procedure as it is currently practiced and provide a better quality of life for patients through the preservation of healthy breast tissue and healthy axial lymph nodes. This proposal will fund the initial evaluation of a fluorescein-cobalamin derivative we now have in hand, and fund the synthesis and evaluation of several second-generation fluorescent cobalamin conjugates that should be brighter and more photostable than our existing compound. Fluorescent cobalamin conjugates will be tested in primary human tumors that are removed from patients undergoing breast cancer operations.