MAGNETIC FIELD DEPENDENCE OF ENZYMES AT WEAK FIELDS

弱场下酶的磁场依赖性

• 批准号: 2019316
• 负责人: CHARLES Buell GRISSOM
• 金额: $ 7.21万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-15 至 1998-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2019316
• 关键词: cytochrome P450; cytochrome c peroxidase; environmental toxicology; enzyme activity; free radicals; magnetic field; methionine; methyltransferase; nitric oxide synthase; radiobiology

DESCRIPTION: The objective of this project is to study the influence of weak DC magnetic fields (0-10 gauss) on several enzymatic reactions that are important in human biochemistry. The heme enzymes nitric oxide synthase, cytochrome P-450, and cytochrome C peroxidase, as well as the B-12 dependent enzyme methionine synthase, are candidates for the site of interaction of 0-10 gauss magnetic fields with biological systems. A magnetic field induced change in the level of nitric oxide, (as produced by nitric oxide synthase), could alter intracellular Ca2+ levels and thereby change Ca2+ efflux rates. This would provide the first causal link between molecular magnetic field effects and a cellular or physiological endpoint of magnetic field exposure. This work could provide a potentially important contribution towards a complete understanding of the possible health effects of environmental magnetic field exposure. The postulated mechanism of interaction is through changes in radical pair recombination, where one radical species is a metal ion (iron in heme, or cobalt in B12).

描述：本项目的目的是研究 弱直流磁场（0-10高斯）对几种酶促反应， 在人类生物化学中很重要 血红素酶一氧化氮合酶， 细胞色素P-450和细胞色素C过氧化物酶，以及B-12依赖性 酶甲硫氨酸合酶，是候选人的网站的相互作用， 0-10高斯磁场与生物系统。 磁场 一氧化氮水平的诱导变化（如由一氧化氮产生 合成酶），可以改变细胞内Ca 2+水平，从而改变Ca 2 + 外排率 这将提供第一个因果关系之间的分子 磁场效应和磁刺激的细胞或生理终点 野外暴露 这项工作可以提供一个潜在的重要 有助于全面了解可能对健康造成的影响 暴露在环境磁场中 假设的机制 相互作用是通过自由基对复合的变化，其中一个 自由基物种是金属离子（血红素中的铁或B12中的钴）。