CORE--TISSUE CULTURE, REAGENTS, AND ELISA

核心B在计划项目中有两个主要角色。核心B将是 负责维持和提供共享的细胞系/cDNA 构建体、融合蛋白、杆状病毒构建体和抗体， 所有参与项目的研究人员。第二，核心将 进行Abeta ELISA以定量总Abeta、Abeta 42（和其它Abeta 42）。 ELISA的，因为他们是发达国家），在及时和方便的方式为所有 参与该项目的研究人员。核心B基本上是 旨在作为一个中央储存库， 大量的高度有用的试剂，已经产生了超过 在过去的十年里，研究人员参与了该项目。这些 包括多种稳定转染的哺乳动物细胞的试剂 细胞系和相应的转基因cDNA构建体，几种融合 蛋白质/杆状病毒构建体和相应的细胞系以及广泛的 收集特异性多克隆和单克隆抗体， 促进持续的合作和整体实验效率 在计划项目中。由于FAD的主要分子后果 早老素基因的突变， Abeta 42：Abeta 40，所有四个项目和转基因核心将需要一个 获得定量测量的有效和方便的手段 Abeta因此，核心B可用于进行Abeta ELISA 任何时候，只要是项目研究者想要的。虽然费用为 维持核心试剂并进行Abeta ELISA（和其他 将进行ELISA，例如早老素，因为它们变得可用） 按核心项目B分列的单个项目费用，包括大规模项目费用- 用于特定生物学实验或蛋白质的细胞系的后处理 净化费用将由个别项目预算支付。核心B 活动将由Tanzi博士和Selkoe博士监督， 核心的运作将由威尔玛·瓦斯科博士监督

Core B will have two primary roles in the Program Project. Core B will be responsible for maintaining and supplying shared cell lines/cDNA constructs, fusion proteins, baculovirus constructs, and antibodies for all investigators involved in the Program Project. Secondly, the Core will perform the Abeta ELISA for quantitating total Abeta, Abeta42 (and other ELISA's as they are developed), in a timely and convenient manner for all investigators involved in the Program Project. Core B is basically intended to serve as a central repository for maintaining and supplying a large number of highly useful reagents that have been generated over the last decade by the Investigators involved in the Program Project. These reagents which include a large variety of stably-transfected mammalian cell lines and corresponding transgene cDNA constructs, several fusion proteins/baculovirus constructs and corresponding cell lines and extensive collection of specific polyclonal and monoclonal antibodies greatly facilitate ongoing collaborations and overall experimental efficiency within the Program Project. Since a major molecular consequence of FAD mutations in the presenilin genes as an increase in the ratio of Abeta42:Abeta40, all four projects and the transgenic core will require an effective and convenient means for obtaining quantitative measurements of Abeta. Therefore, Core B will be available to carry out the Abeta ELISA whenever desired by a Program Investigator. While the expenses for maintain the Core reagents and performing the Abeta ELISA (and other ELISAs, e.g. for presenilins, as they become available) will be incurred by Core B, individual project expenses including those for large scale- workup of cell lines for specific biological experiments or protein purifications will be covered by individual project budgets. Core B activities will be overseen by Dr. Tanzi and Dr. Selkoe while day-to-day operations of the Core will be supervised by Dr. Wilma Wasco.