CORE--CELL CULTURE FACILITY

组织库核心设施将参与采集 以及将前列腺组织分配给孢子赠与者的成员。 组织银行核心基金有以下目标：(1)最大限度地 收获的组织数量，同时仍能实现 标本的病理检查。(2)获取纯净的组织 可能(即由75%的肿瘤核组成的肿瘤，或良性 前列腺组织未受大体看不见的污染 浸润性癌症。(3)获得广谱的肿瘤 分级、分期和组织学亚型。(4)获取不同类型的 良性前列腺组织(移行区和外周区)。(5) 采集含有可能的前列腺癌前驱病变的组织 癌症(前列腺上皮内瘤变)。(六)协调 将组织分配给孢子授予的不同成员和 最终传给该机构以外的研究人员。每个人的遗嘱 包括肿瘤、良性周边区、良性移行区、 精囊和肿瘤附近的组织。新技术 已经被开发用来采集最大量的组织 而不影响标本的病理评估。 准确的病理分期和手术切缘评估 将是辅助治疗试验和 不同研究参数与肿瘤的相关性研究 生物行为。前列腺及相关组织的冰冻标本 从总共658名正在接受治疗的个体中收集到 自1995年1月12日起在约翰·霍普金斯医院接受手术。1993年，我们成立了 研究人员可以通过这个系统获得新鲜的、无菌的前列腺 组织，以及冰冻的前列腺组织。因为只有 相对少量的癌症组织可以被采集用于 研究使用来自每个前列腺的组织提供给研究人员 在加权轮换的基础上。使用该系统，381个新鲜组织 已将样本提供给187个人中的14个人 自从我们开始跟踪这件事以来，我们就分开了病人 前列腺组织库样本编号338。已优先考虑 病毒转导研究因为他们需要大量的 肿瘤样本，其余研究人员大致持平。 冷冻组织已被用于突变研究，等位基因丢失 研究，原位杂交研究，原位聚合酶链式反应研究 免疫化学研究和端粒酶研究 十)独立的研究小组。

The Tissue Bank Core Facility will be involved in the harvesting and distribution of prostatic tissue to members of the SPORE grant. The Tissue Bank Core Facility has the following aims: (1) Maximize the amount of tissue harvested while still enabling thorough pathologic assessment of the specimen. (2) Obtain as pure tissue as possible (i.e. tumor composed of >75% tumor nuclei, or benign prostate tissue without contamination by grossly inapparent infiltrating cancer. (3) Obtain tumors with a wide spectrum of grade, stage and histologic subtype. (4) Obtain different types of benign prostate tissue (transitional and peripheral zone). (5) Harvest tissue containing putative precursor lesions of prostate cancer (prostatic intraepithelial neoplasia). (6) Coordinate the distribution of tissue to varies members of the SPORE grant and eventually to researchers outside of the institution. Each will consist of tumor, benign peripheral zone, benign transition zone, seminal vesicles, and tissue adjacent to tumor. Novel techniques have been developed to harvest the greatest amount of tissue without compromising the pathologic assessment of the specimen. Accurate pathologic staging and evaluation of margins of resection will be necessary for adjuvant therapeutic trials and for correlation of various investigational parameters with tumor biologic behavior. Frozen samples of prostate and related tissue have been collected from a total of 658 individuals undergoing surgery at Johns Hopkins Hospital as of 1/12/95. In 1993 we set up a system whereby researchers can obtain fresh, sterile prostate tissue, as well as frozen prostate tissue. Because only a relatively small amount of cancer tissue can be harvested for research use from each prostate, tissue is provided to researchers on a weighted rotating basis. Using this system, 381 fresh tissue samples have been provided to 14 separate individuals from 187 separate patients since we began keeping track of this with prostate tissue bank sample number 338. Priority has been given to the viral transduction studies because of their need for large tumor samples, with the remaining researchers roughly at par. Frozen tissue has been used for mutation studies, allelic loss studies, in situ hybridization studies, in situ PCR studies, immunochemistry studies, and telomerase studies by several (over ten) separate research groups.