PDX-1 IN MAMMALIAN PANCREATIC DEVELOPMENT
PDX-1 在哺乳动物胰腺发育中的作用
基本信息
- 批准号:6105437
- 负责人:
- 金额:$ 22.99万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1999
- 资助国家:美国
- 起止时间:1999-07-01 至 2000-06-30
- 项目状态:已结题
- 来源:
- 关键词:cell differentiation developmental genetics duodenum gene expression gene targeting genetic enhancer element genetic promoter element genetic regulatory element genetically modified animals histogenesis homeobox genes laboratory mouse lung pancreas pancreatic islets reporter genes transcription factor
项目摘要
Description: (Directly taken from the application) The PDX-l gene
(pancreas-duodenum homeobox gene - previously called X1Hbox8 in Xenopus
(Wright et al., 1988), Idx-l or Stf-l in rat (Miller et al., 1994;
Leonard et al., 1994), and Ipf-l in mouse (Ohlsson et al., 1994)) is
expressed in the endoderm of frogs and mice in the developing rostral
duodenum and pancreas. It is known that mice homozygous for a null
mutation of the PDX- l gene have no pancreas and die as neonates. PDX-I
thus joins the orphan homeobox gene spx, (previously called Hox-11;
Roberts et al., 1994) being required for development of a specific organ.
In mature murine pancreatic islets, PDX-I protein is specifically
expressed in nuclei of beta cells. We have generated evidence that PDX-1
is part of the transcription factor complex activating insulin
transcription, through the mutationally sensitive and evolutionarily
conserved promoter sequence termed Flat-E. We propose a set of
approaches to address the role of PDX-l in endodermal regional
differentiation, the mechanism behind its region-specific activation in
the pancreatic/duodenal area, and its role in islet cell ontogeny. We
will assess the effects of mis-expressing PDX-1 in early developing lung
epithelium and of sustaining its expression in acinar (exocrine) cells,
in which it is not normally expressed. We will undertake a promoter
analysis of the PDX-1 gene in vivo using reporter gene constructs in
transgenic mice to find promoter/enhancer modules responsible for its
normal expression pattern. Lastly, we will begin to address the role of
PDX-1 in islet differentiation by examining the development of pancreatic
tissue after cell type-specific gene excision using Cre-lox P system, and
in chimeric PDX-1-/-/wild type animals.
描述:(直接取自申请书)pdx - 1基因
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Christopher V Wright其他文献
Christopher V Wright的其他文献
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{{ truncateString('Christopher V Wright', 18)}}的其他基金
Control of endocrine pancreatic beta-cell fate, function, and proliferation
控制内分泌胰腺 β 细胞的命运、功能和增殖
- 批准号:
10359799 - 财政年份:2018
- 资助金额:
$ 22.99万 - 项目类别:
Architecture and communication controlling the efficient generation of beta cells
控制β细胞有效生成的架构和通信
- 批准号:
8316317 - 财政年份:2010
- 资助金额:
$ 22.99万 - 项目类别:
Architecture and communication controlling the efficient generation of beta cells
控制β细胞有效生成的架构和通信
- 批准号:
8143507 - 财政年份:2010
- 资助金额:
$ 22.99万 - 项目类别:
Architecture and communication controlling the efficient generation of beta cells
控制β细胞有效生成的架构和通信
- 批准号:
8522280 - 财政年份:2010
- 资助金额:
$ 22.99万 - 项目类别:
Architecture and communication controlling the efficient generation of beta cells
控制β细胞有效生成的架构和通信
- 批准号:
8717653 - 财政年份:2010
- 资助金额:
$ 22.99万 - 项目类别:
Architecture and communication controlling the efficient generation of beta cells
控制β细胞有效生成的架构和通信
- 批准号:
7994960 - 财政年份:2010
- 资助金额:
$ 22.99万 - 项目类别:
PDX-1 IN MAMMALIAN PANCREATIC DEVELOPMENT
PDX-1 在哺乳动物胰腺发育中的作用
- 批准号:
6466603 - 财政年份:2001
- 资助金额:
$ 22.99万 - 项目类别:
CONTROL OF CORNEAL ENDOTHELIUM DEVELOPMENT IN THE MOUSE
小鼠角膜内皮发育的控制
- 批准号:
6530102 - 财政年份:2000
- 资助金额:
$ 22.99万 - 项目类别:
PDX-1 IN MAMMALIAN PANCREATIC DEVELOPMENT
PDX-1 在哺乳动物胰腺发育中的作用
- 批准号:
6352881 - 财政年份:2000
- 资助金额:
$ 22.99万 - 项目类别:
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