SALIVARY ARGININE/LYSINE/PEPTIDES AND CARIES EXPERIENCE

在无龋和有龋之间观察到的最一致的差异是， 易感受试者与菌斑pH值调节有关;无龋齿 个人更有效地中和牙菌斑酸。我们假设 这种差异与游离二元氨基酸水平升高有关 和精氨酸/赖氨酸的肽在唾液中的无龋 人士 我们将首先定量单个低分子量 从成熟的无龋和龋的腮腺唾液中收集的肽， 易感成年人（等于或大于50岁）。唾液肽 观察到与龋齿经历相关的， 其特征在于它们能够缓冲菌斑流体并产生 当被口腔微生物代谢时氨。我们还将提高 与龋齿相关的唾液肽抗体 经验的目的，发展免疫测定，以定量这些 缩氨酸然后，我们将确定一组中这些肽的水平， 将被纵向检查四年的儿童;在每个 检查，龋齿状态（即无龋齿或龋齿敏感）将被 测定腮腺唾液肽和游离氨基酸。 牙菌斑pH相关唾液因子的鉴定 调节将提高我们对宿主对牙齿抗性的理解 龋齿

The most consistent difference observed between caries-free and caries- susceptible subjects relates to regulation of plaque pH; caries free individuals neutralize plaque acid more effectively. We hypothesize that this difference is related to elevated levels of free dibasic amino acids and arginine/lysine-containing peptides in the saliva of caries-free persons. We will first quantitate levels of individual low molecular weight peptides in parotid saliva collected from mature caries-free and caries- susceptible adults (equal to or more than 50 yrs.). Salivary peptides which are observed to be associated with caries experience will be characterized for their ability to buffer plaque fluid and to yield ammonia when metabolized by oral microorganisms. We will also raise antibodies to the salivary peptides which are associated with caries experience for the purpose of developing immunoassays to quantitate these peptides. We will then determine the levels of these peptides in a group of children who will be examined longitudinally for four years; at each exam, caries status (i.e. caries-free or caries-susceptible) will be determined and parotid salivary peptides and free amino adds quantitated. The identification of salivary factors which are involved in plaque pH regulation will enhance our understanding of host resistance to dental caries.