SALIVARY ARGININE/LYSINE/PEPTIDES AND CARIES EXPERIENCE

在无龋齿和龋齿之间观察到的最一致的区别是- 易感人群与调节菌斑pH值有关；无龋齿 个体更有效地中和斑块酸。我们假设 这种差异与游离二元氨基酸水平升高有关。 和无龋者唾液中的精氨酸/赖氨酸多肽 人。 我们将首先对个体低分子量的水平进行量化 腮腺唾液中的多肽来自成熟的无龋者和- 易感成人(等于或超过50岁)。唾液肽 观察到哪些与龋齿有关的经验将是 其特点是能够缓冲斑块液体并产生 被口腔微生物代谢时的氨。我们还将筹集 与龋齿有关的唾液肽抗体 在开发免疫分析方法以定量这些方面的经验 多肽。然后我们将确定这些多肽在一组中的水平 将接受四年纵向检查的儿童；每名儿童 检查，龋齿状态(即无龋齿或易龋齿)将为 测定腮腺唾液肽和游离氨基酸的含量。 影响菌斑pH的唾液因素的鉴定 监管将加强我们对宿主对牙科疾病抵抗力的了解 龋齿。

The most consistent difference observed between caries-free and caries- susceptible subjects relates to regulation of plaque pH; caries free individuals neutralize plaque acid more effectively. We hypothesize that this difference is related to elevated levels of free dibasic amino acids and arginine/lysine-containing peptides in the saliva of caries-free persons. We will first quantitate levels of individual low molecular weight peptides in parotid saliva collected from mature caries-free and caries- susceptible adults (equal to or more than 50 yrs.). Salivary peptides which are observed to be associated with caries experience will be characterized for their ability to buffer plaque fluid and to yield ammonia when metabolized by oral microorganisms. We will also raise antibodies to the salivary peptides which are associated with caries experience for the purpose of developing immunoassays to quantitate these peptides. We will then determine the levels of these peptides in a group of children who will be examined longitudinally for four years; at each exam, caries status (i.e. caries-free or caries-susceptible) will be determined and parotid salivary peptides and free amino adds quantitated. The identification of salivary factors which are involved in plaque pH regulation will enhance our understanding of host resistance to dental caries.