CORE--CHIMERISM ANALYSIS AND HISTOCOMPATIBILITY TYPING

描述：（申请人描述）本核心项目旨在满足本项目项目资助中所有三个研究项目的需求。核心有两个组成部分。一个将提供服务，另一个将开发未来技术。服务组件寻求实现两个目标。一个目标是利用微卫星标记多态性在狗和人类患者干细胞移植后提供嵌合分析。这些用于评估在所有三个研究项目下进行的研究中的造血移植。第二个目标是为项目1和项目3提供犬组织相容性分型（犬白细胞抗原分型），目的是选择供-受体对进行移植。该研究部分将用于项目1和项目2的未来研究，这些研究将包括与主要犬组织相容性复合体（MHC）抗原匹配的非相关供体-受体配对。核心的研究部分也追求两个目标。第一个涉及到聚合酶链反应-单链构象多态性测定的发展，用于分型多态的第一类基因（已经对第二类基因进行了分型）。第二个目标涉及筛选细菌人工染色体（BAC）文库，以定位先前未表征的多态MHC基因，这些基因可能在无关干细胞移植后的宿主抗移植物和移植物抗宿主反应中发挥重要作用。

DESCRIPTION: (Applicant's Description) This Core is designed to meet the needs of all three research projects on this program project grant. The core has two components. One will provide service and the other will develop future technologies. The service component seeks to accomplish two goals. One goal is to provide chimerism analyses in dogs and human patients after stem cell transplantation using microsatellite marker polymorphism. These serve to evaluate hematopoietic engraftation in studies carried out under all three research projects. The second goal is to provide canine histocompatibility typing (Dog leukocyte Antigen typing) for Projects 1 and 3 with the aim of selecting littermate donor-recipient pairs for transplantation. The research component will serve future studies in projects 1 and 2 which will include unrelated donor-recipient pairs that are matched for the major canine histocompatibility complex (MHC) antigens. The research component of the core also pursues two goals. The first involves the development of a polymerase chain reaction-single strand conformational polymorphism assay for typing polymorphic clas I genes (as has already been done for class II genes). The second goal involves screening a Bacterial Artificial Chromosome (BAC) library to locate previously uncharacterized polymorphic MHC genes which may play important roles in host-versus-graft and graft-versus-host reactions after unrelated stem cell transplantation.