CORE--CHIMERISM ANALYSIS AND HISTOCOMPATIBILITY TYPING

核心——嵌合分析和组织相容性分型

• 批准号: 6103526
• 负责人: JOHN M WAGNER
• 金额: $ 24.18万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-12 至 2000-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6103526
• 关键词: artificial chromosomes; biomedical facility; dogs; graft versus host disease; hematopoietic stem cells; hematopoietic tissue transplantation; histocompatibility antigens; histocompatibility typing; human subject; tissue mosaicism

DESCRIPTION: (Applicant's Description) This Core is designed to meet the needs of all three research projects on this program project grant. The core has two components. One will provide service and the other will develop future technologies. The service component seeks to accomplish two goals. One goal is to provide chimerism analyses in dogs and human patients after stem cell transplantation using microsatellite marker polymorphism. These serve to evaluate hematopoietic engraftation in studies carried out under all three research projects. The second goal is to provide canine histocompatibility typing (Dog leukocyte Antigen typing) for Projects 1 and 3 with the aim of selecting littermate donor-recipient pairs for transplantation. The research component will serve future studies in projects 1 and 2 which will include unrelated donor-recipient pairs that are matched for the major canine histocompatibility complex (MHC) antigens. The research component of the core also pursues two goals. The first involves the development of a polymerase chain reaction-single strand conformational polymorphism assay for typing polymorphic clas I genes (as has already been done for class II genes). The second goal involves screening a Bacterial Artificial Chromosome (BAC) library to locate previously uncharacterized polymorphic MHC genes which may play important roles in host-versus-graft and graft-versus-host reactions after unrelated stem cell transplantation.

描述：（申请人的描述）本核心旨在满足本计划项目资助的所有三个研究项目的需求。 核心有两个组成部分。 一个将提供服务，另一个将开发未来的技术。 服务组件寻求实现两个目标。 一个目标是提供嵌合体分析在狗和人类患者干细胞移植后，使用微卫星标记多态性。这些用于评估在所有三个研究项目下进行的研究中的造血移植。 第二个目标是为项目1和3提供犬组织相容性分型（犬白细胞抗原分型），目的是选择同窝供体-受体对进行移植。研究部分将服务于项目1和2的未来研究，其中将包括与主要犬组织相容性复合体（MHC）抗原相匹配的无关供体-受体对。 核心的研究部分也追求两个目标。 第一个涉及的发展的聚合酶链反应-单链构象多态性分析分型多态性I类基因（已经完成了II类基因）。 第二个目标是筛选细菌人工染色体（BAC）库，以定位以前未表征的多态性MHC基因，这些基因可能在无关干细胞移植后的宿主抗移植物和移植物抗宿主反应中发挥重要作用。