BIACORE-2000 SURFACE PLASMON RESONANCE SYSTEM

本提案所要求的表面等离子体共振仪(Biacore 2000)将用于测量各种系统中生物分子相互作用的动力学常数和平衡常数。这些系统涉及蛋白质/蛋白质相互作用(T细胞受体/多肽MHC或超抗原配体；工程单链抗体/T细胞受体；趋化受体/信号转导蛋白；细胞黏附蛋白E-钙粘素；T细胞受体CD4/CD48)；蛋白质/核酸相互作用(雌激素受体/DNA识别序列；Viglin/RNA识别序列)；以及蛋白质/脂质相互作用(血浆蛋白质/脂蛋白；载脂蛋白/磷脂表面)。促成这一提议的六名研究人员来自伊利诺伊大学生物化学和化学工程系，他们是生物技术中心的成员。表面等离子体共振仪将是这个校园里第一台这样的仪器。它将设在生物技术中心的免疫资源中心，负责该仪器的维护和日常管理。

The surface plasmon resonance instrument (BIAcore 2000) requested in this proposal will be used to measure the kinetic and equilibrium constants of biomolecular interactions in a variety of systems. The systems involve protein/protein interactions (T-cell receptor/peptide MHC or super-antigen ligands; engineered single chain antibodies/T-cell receptor; chemotaxis receptor/signal transducing proteins; homotropic interactions of the cell adhesion protein E-cadherin; T-cell receptor CD4/CD48); protein/nucleic acid interactions (estrogen receptor/DNA recognition sequences; vigilin/RNA recognition sequences); and protein/lipid interactions (plasma proteins/lipoproteins; apolipoproteins/phospholipid surfaces). The six investigators contributing to this proposal come from the Departments of Biochemistry and Chemical Engineering of the University of Illinois and are members of the Biotechnology Center. The surface plasmon resonance instrument will be the first such instrument on this campus. It will be located in the Immunological Resource Center of the Biotechnology Center which will assume the responsibility for the maintenance, and day-to-day administration of this instrument.