SPECTROPOLARIMETER--JASCO MODEL J-600

本提案中要求的光谱偏振仪(JASCOJ-600型) 将用于测量圆二向色性(CD)和磁性圆 在二级结构研究中的二色性(MCD)谱， 动力学，以及各种蛋白质的相互作用。这些系统将是 研究包括溶液和脂质中的人血浆载脂蛋白 大肠埃希菌和红细菌的电子传递酶结合态 假单胞菌中的球状杆菌、恶臭还蛋白和细胞色素P450CAM 恶臭，哺乳动物视紫红质和转导蛋白，以及细菌视紫红质来自 盐生杆菌和人血浆中的脂转移蛋白。 促成这一提议的调查人员来自几个 伊利诺伊大学香槟分校系，包括 生物化学、物理学、生理学和生物物理学，以及食品科学。这 基础广泛的利益反映了这样一个事实，即没有一个 该校园提供可操作的CD仪器。这件CD乐器 将由化学科学学院维护，并将位于 在荧光动力学实验室(LFD)。

The spectropolarimeter (JASCO Model J-600) requested in this proposal will be used to measure circular dichroism (CD) and magnetic circular dichroism (MCD) spectra in the investigation of the secondary structure, dynamics, and interactions of various proteins. The systems to be studied include human plasma apolipoproteins in solution and in lipid bound states, the electron transport enzymes of E. coli and Rhodobacter sphaeroides, putidaredoxin and cytochrome P450CAM from Pseudomonas putida, mammalian rhodopsin and transducin, and bacteriorhodopsin from Halobacterium halobium, and the lipid transfer protein from human plasma. The investigators contributing to this proposal come from several departments of the University of Illinois at Urbana-Champaign, including Biochemistry, Physics, Physiology and Biophysics, and Food Science. This broad based interest reflects the fact that there is not a single operational CD instrument available on this campus. This CD instrument will be maintained by the School of Chemical Sciences and will be located in the Laboratory for Fluorescence Dynamics (LFD).