SPECTROPOLARIMETER--JASCO MODEL J-600

分光偏振仪--JASCO MODEL J-600

• 批准号: 3520307
• 负责人: ANA JONAS
• 金额: $ 8.7万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-06-12 至 1990-06-11
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3520307
• 关键词: apolipoproteins; bacterial proteins; bacteriorhodopsins; biomedical equipment purchase; circular dichroism; circular magnetic dichroism; cytochrome P450; polarimetry; protein structure; transport proteins

The spectropolarimeter (JASCO Model J-600) requested in this proposal will be used to measure circular dichroism (CD) and magnetic circular dichroism (MCD) spectra in the investigation of the secondary structure, dynamics, and interactions of various proteins. The systems to be studied include human plasma apolipoproteins in solution and in lipid bound states, the electron transport enzymes of E. coli and Rhodobacter sphaeroides, putidaredoxin and cytochrome P450CAM from Pseudomonas putida, mammalian rhodopsin and transducin, and bacteriorhodopsin from Halobacterium halobium, and the lipid transfer protein from human plasma. The investigators contributing to this proposal come from several departments of the University of Illinois at Urbana-Champaign, including Biochemistry, Physics, Physiology and Biophysics, and Food Science. This broad based interest reflects the fact that there is not a single operational CD instrument available on this campus. This CD instrument will be maintained by the School of Chemical Sciences and will be located in the Laboratory for Fluorescence Dynamics (LFD).

本提案中要求的分光偏振计（JASCO Model J-600） 将用于测量圆二色性（CD）和磁性圆二色性（CD）。 二色性（MCD）光谱在二级结构的研究中， 动力学和各种蛋白质的相互作用。 系统将 研究包括溶液和脂质中的人血浆载脂蛋白 结合态，E.大肠杆菌和红杆菌 假单胞菌属的类球蛋白、putidaredoxin和细胞色素P450 CAM putida，哺乳动物视紫红质和转导素，以及来自 盐生盐杆菌和来自人血浆的脂质转移蛋白。 为这项提议做出贡献的调查人员来自几个 伊利诺伊大学厄巴纳-香槟分校的部门，包括 生物化学、物理学、生理学和生物物理学以及食品科学。 这 广泛的兴趣反映了这样一个事实，即没有一个单一的 可以使用的CD设备 这张CD 将由化学科学学院维护，并将位于 荧光动力学实验室（LFD）