ABI PRISM 7700 SEQUENCE DETECTION SYSTEM

ABI PRISM 7700 序列检测系统

• 批准号: 2803489
• 负责人: CLIONA M ROONEY
• 金额: $ 10.14万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2803489
• 关键词: biomedical equipment purchase; clinical biomedical equipment; computer assisted sequence analysis; nucleic acid sequence; polymerase chain reaction

This is an application for funds to purchase an ABI PRISM 7700 Sequence Detection System for use by three NIH funded clinical researchers to monitor patients enrolled in gene transfer studies. Detection of DNA or RNA by real time PCR using this machine has several advantages over conventional semi-quantitative PCR in providing increases sensitivity and specificity as well as reducing the time of the assay and increasing safety by eliminating the requirement for radioactive probing. The projects described by the investigators within this application would therefore be able to address issues of quantitation of transgene expression with a much higher degree of accuracy and facility, improving the safety and increasing the information to be gained from the clinical research studies.

这是一份资金申请，用于购买ABI PRISM 7700序列检测系统，供三名NIH资助的临床研究人员使用，以监测参与基因转移研究的患者。使用该机器通过真实的时间PCR检测DNA或RNA与常规半定量PCR相比具有几个优点，即提供增加的灵敏度和特异性，以及减少测定时间和通过消除对放射性探测的要求而增加安全性。因此，研究人员在本申请中描述的项目将能够以更高的准确性和便利性解决转基因表达定量问题，提高安全性并增加从临床研究中获得的信息。