PURCHASE OF A MALDI-TOF MASS SPECTROMETER

购买 MALDI-TOF 质谱仪

• 批准号: 2791791
• 负责人: KENNETH J SHEA
• 金额: $ 25万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2001-04-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2791791
• 关键词: biomedical equipment; biomedical equipment purchase; matrix assisted laser desorption ionization

Support for purchasing matrix assisted laser desorption ionization time of flight mass spectrometer (MALDI-TOF-MS) which is essential to many NIH-funded research programs is requested. No MALDI-TOF-MS is available on the UC Irvine campus. Current research is being hindered by the lack of a MALDI-TOF system. This was demonstrated by the productivity gains that were made, and the experience gained, during the loan of a requested instrument is vital to the interests of three Schools at UCI: Physical Sciences, Biological Sciences and Medicine and will therefore be truly a shared inter-disciplinary instrument. Research projects of the major users include: (a) Shea, novel polymers, co-polymers, block polymers and ionomers, (b) Chamberlin, binding site determination for neuronal glutamate receptors; (c) Feher, novel dendritic compounds with peptidic and carbohydrate functionalities on a silsesquioxane core; (d) Glabe, amyloid protein and peptide structural characterization for Alzheimer's disease research; (e) Henschen, structure/function studies on normal and variant forms of fibrinogen; (f) Selsted, characterization and activity of novel peptidic antimicrobial agents; (g) Van Vranken, studies on chemical processes in protein aging. Aside from the major user group there are many other NIH funded research group who urgently require MALDI-TOF capability. The nature of the instrument to be purchased is such that it can be readily operated by the researcher but also that it will be fully exploited by placing it in a mass spectrometry facility with facility director who has over 20 years experience in mass spectrometry. The UCI mass spectrometry facility which comprises of two magnetic sector instruments is fully saturated providing analytical support for synthetic program (extract mass determinations) and other problem solving, particularly using LSIMS, direct probe, GC-MS and FD. The sample load for the two magnetic sector instruments is over 3,500 per year with the vast majority requiring extract mass determinations. UCI has a long-term major funding commitment to the mass spectrometry facility as well as the other analytical spectroscopy instrumentation facilities which support the research faculty. Currently the annual commitment is over $500,000 to these facilities in eight salaries and operational support. Additional operation funding is obtained through charges to the grants and contacts of the user community.

要求支持购买基质辅助激光解吸电离飞行时间质谱仪（MALDI-TOF-MS），这对许多NIH资助的研究计划至关重要。没有MALDI-TOF-MS在UC Irvine校园可用。目前的研究受到缺乏MALDI-TOF系统的阻碍。这是由所取得的生产力的提高，并获得了经验，在贷款期间所要求的仪器是至关重要的三个学校在UCI的利益：物理科学，生物科学和医学，因此将是一个真正的共享跨学科的工具。主要用户的研究项目包括：（a）乳木果，新型聚合物、共聚物、嵌段聚合物和离聚物;（B）Chamberlin，神经元谷氨酸受体的结合位点测定;（c）Feher，在倍半硅氧烷核上具有肽和碳水化合物功能的新型树枝状化合物;（d）Glabe，淀粉样蛋白和肽结构表征，用于阿尔茨海默病研究;（e）Henschen，关于正常和变异形式的纤维蛋白原的结构/功能研究;（f）Selsted，关于新的肽类抗菌剂的特性和活性;（g）货车Vranken，关于蛋白质老化的化学过程的研究。除了主要的用户群之外，还有许多其他NIH资助的研究小组迫切需要MALDI-TOF功能。拟购买的仪器的性质是，研究人员可以很容易地操作它，但也可以通过将它放置在质谱设施中，由具有20多年质谱经验的设施主任充分利用它。由两台磁扇区仪器组成的UCI质谱设施完全饱和，为合成程序（提取物质量测定）和其他问题解决提供分析支持，特别是使用LSIMS，直接探针，GC-MS和FD。两个磁扇区仪器的样品负载每年超过3，500个，其中绝大多数需要提取物质量测定。UCI对质谱设施以及支持研究人员的其他分析光谱仪器设施有长期的主要资助承诺。目前，每年向这些设施提供50多万美元的薪金和业务支助。通过向用户社区的赠款和联系人收费获得额外的业务资金。