FLUORESCENCE ACTIVATED CELL SORTER

荧光激活细胞分选仪

• 批准号: 2791051
• 负责人: KEITH E WEAVER
• 金额: $ 2.51万
• 依托单位: UNIVERSITY OF SOUTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-15 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2791051
• 关键词: biomedical equipment; biomedical equipment purchase; flow cytometry

The instrumentation requested in this proposal is a fluorescence- activated cell sorter (FACS) system. A FACS uses laser technology to examine fluorescently tagged intracellular and cell surface proteins and DNA content of live or fixed individual cells (FACS analysis). In addition, the equipment is designed to sort cells with fluorescently tagged proteins and/or DNA content (FACS sorting). The major component of the system are the laser bench and optics, a UV and an argon laser, detectors, controller, and computer/software/printer. The common thread running through the research described in this proposal is the need for analyzing and sorting single cells; however, the areas of biomedical research requiring cell analysis/cell sorting are diverse. NIH-funded projects under investigation at U.S.D. which would benefit from better access to a FACS include studies of: . Assembly of class I major histocompatibility complex molecules (which present antigens to immune T cells), and interference with this assembly process by virus proteins . Effects of lentiviruses on equine and human cells. . Mechanisms responsible for tissue-specific, differential aldehyde dehydrogenase gene expression, which is inducible in the liver upon exposure to toxins and during carcinogenesis. . Coupling of exit from the cell cycle to differentiation for oligodendrocytes (the cells in the central nervous system which produce myelin) . Alterations in cardiac myocyte proteins during cardiac hypertrophy and congestive heart failure

本提案中要求的仪器是荧光激活细胞分选仪（FACS）系统。FACS使用激光技术检查活的或固定的单个细胞的荧光标记的细胞内和细胞表面蛋白质和DNA含量（FACS分析）。此外，该设备设计用于分选具有荧光标记蛋白质和/或DNA含量的细胞（FACS分选）。该系统的主要组成部分是激光工作台和光学器件、紫外和氩激光器、探测器、控制器和计算机/软件/打印机。贯穿本提案所述研究的共同主线是需要分析和分选单细胞;然而，需要细胞分析/细胞分选的生物医学研究领域是多种多样的。NIH资助的项目正在美国研究。更好地利用外地资产管制系统将使其受益的研究包括：I类主要组织相容性复合体分子（将抗原呈递给免疫T细胞）的组装，以及病毒蛋白对该组装过程的干扰。慢病毒对马和人类细胞的影响。.负责组织特异性、差异性醛脱氢酶基因表达的机制，其在暴露于毒素和致癌过程中在肝脏中是可诱导的。.从细胞周期退出到少突胶质细胞（中枢神经系统中产生髓鞘的细胞）分化的耦合。心肌肥厚和充血性心力衰竭时心肌细胞蛋白质的变化