ABI PRISM MODEL 377 DNA SEQUENCER

ABI Prism 377自动DNA测序仪的申请是由一组NIH支持的研究人员提交的，他们将DNA测序作为其研究工作的重要组成部分。他们代表着一个由128名主要研究人员组成的基础广泛的用户群体，其中大多数是NIH的受赠人，他们的研究严重依赖于具有成本效益和成本效益的DNA测序，以表征自然种群、诱导和自发突变、NAD实验室结构中的DNA变异和突变。应用于基础和应用生物医学研究，包括对人类、狗、小鼠、果蝇、酵母、线虫、病毒和植物的研究。目前，一台ABI 377自动DNA测序仪通过伊萨卡的DNA测序通用设施提供，为伊萨卡校区和纽约城市医学院提供服务。它目前的容量为81%(每月超过2600个序列)，按照目前的增长速度，将在三个月内(到1998年6月)达到最大容量。这带来了两个问题。随着产能的达到，周转时间将增加，从而推迟研究。其次，在机器停机时，所有DNA测序都会关闭。该设施需要第二台ABI Prism 377 DNA测序仪，以缓解这种情况，并使康奈尔生物资源中心更接近为这一大群研究人员提供高通量DNA测序和基因分型的适当标准。

This application for an ABI Prism 377 Automated DNA Sequencer is being submitted by a group of NIH supported investigators who use DNA sequencing as a significant part of their research effort. They represent a broad-based user group of 128 principal investigators, the majority of which are NIH grantees, whose research depends critically on cost efficient and cost effective DNA sequencing to characterize DNA variation and mutations in natural populations, induced and spontaneous mutants, nad laboratory constructs. Applications are in basic and applied biomedical research including work on humans, dogs, mice, Drosophila, yeast, nematodes, viruses, and plants. Currently, a single ABI 377 automated DNA sequencer is available through a common-use facility for DNA sequencing in Ithaca, serving both the Ithaca campus and the NY City Medical College. It is currently at 81% capacity (more than 2600 sequences per month), and at current rate of growth, will be at maximum capacity within three months (by June 1998). This presents two problems. As capacity is reached, turn-around time will increase, delaying research. Second, in times of machine down-time, all DNA sequencing is shut down. A second ABI Prism 377 DNA Sequencer is requested for this facility to alleviate this situation and move Cornell's BioResource Center closer to an appropriate standard for high throughput DNA sequencing and genotyping for this large group of investigators.