BIACORE 2000 PROCESSING UNIT

BIACORE 2000 处理单元

基本信息

批准号：
2766833
负责人：
UTTAM L RAJBHANDARY
金额：
$ 23.8万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-03-15 至 2000-03-14
项目状态：
已结题

项目摘要

The application by five P.I.'s proposes to use surface plasmon resonance for the kinetic and thermodynamic analysis of specific protein-protein and protein-nucleic acid interactions in a variety of biological systems. The RAJBHANDARY laboratory will perform studies of protein synthesis initiation in eubacteria and in eukaryotes. The molecular mechanisms underlying the specific interaction between the E. coli and human initiator transfer RNAs and the corresponding protein synthesis initiation factors IF2 and eIF2 will be studied using wild type and mutant initiator transfer RNAs. The KHORANA laboratory will study binding and kinetic constants of protein-protein interactions in visual processes (sensitization and desensitization) following light-activation of the vertebrate photoreceptor rhodopsin. Initial focus will be on interactions between activated receptor and the G protein, transducin, on the one hand and between the receptor and rhodopsin kinase on the other. The KIM laboratory will study peptides from the core of HIV gp41, that are known to be inhibitors of HIV infection. The kinetics of association and dissociation of these peptides, and derivatives of these peptides, will be investigated in order to provide information to assist the development of drugs to treat AIDS. The KRIEGER laboratory will study cell surface scavenger receptors which bind chemically modified lipoproteins and have been implicated in atherogenesis, in the recognition and clearance of apoptotic cells and toxic and pathogenic substances, and in HDL metabolism. BIACORE instrument will be used to explore the complex binding properties of these diverse, multiligand receptors. The SAUER laboratory is engaged in studies of (i) repressor- DNA interactions, (ii) how intracellular proteases recognize specific sequences in proteins targeted for destruction and (iii) factors that influence homo versus heterodimerization of the Arc repressor and its variants.

五个P.I.的应用建议将表面等离子体共振用于多种生物系统中特定蛋白质蛋白质和蛋白核酸相互作用的动力学和热力学分析。 Rajbhandary实验室将对精杆菌和真核生物中的蛋白质合成起始进行研究。将使用野生型和突变引发剂转移RNA研究，将研究大肠杆菌与人类引发剂转移RNA与相应蛋白质合成起始因子IF2和EIF2之间特定相互作用的分子机制。 Khorana实验室将研究脊椎动物光感受器动蛋白的光激活后，在视觉过程中（致敏和脱敏）中蛋白质 - 蛋白质相互作用的结合和动力学常数。最初的焦点将一方面，一方面以及受体和若云蛋白激酶之间的激活受体和G蛋白之间的相互作用。 KIM实验室将研究HIV GP41核心的肽，这些肽已知是HIV感染的抑制剂。这些肽的关联和解离动力学以及这些肽的衍生物将进行研究，以提供信息以帮助开发药物以治疗艾滋病。 Krieger实验室将研究结合化学修饰的脂蛋白的细胞表面清道夫受体，并与动脉粥样硬化有关，识别和清除凋亡细胞，有毒和致病性物质以及HDL代谢。 BIACORE仪器将用于探索这些多样的多发受体的复杂结合特性。 SAUER实验室参与了（i）抑制剂 - DNA相互作用的研究，（ii）细胞内蛋白酶如何识别靶向破坏的蛋白质中的特定序列以及（iii）影响HOMO与ARC抑制剂及其变体异二聚体的因素。