BIACORE 2000 PROCESSING UNIT

五名私家侦探的申请等人提出使用表面等离子体共振对多种生物系统中的特定蛋白质-蛋白质和蛋白质-核酸相互作用进行动力学和热力学分析。 RAJBHANDARY实验室将进行真细菌和真核生物中蛋白质合成起始的研究。 探讨了E.将使用野生型和突变型起始转移RNA研究大肠杆菌和人起始转移RNA以及相应的蛋白质合成起始因子IF 2和eIF 2。 KHORANA实验室将研究脊椎动物光感受器视紫红质光激活后视觉过程（敏化和脱敏）中蛋白质-蛋白质相互作用的结合和动力学常数。最初的重点将是激活受体和G蛋白，转导蛋白，一方面和受体和视紫红质激酶之间的相互作用。 KIM实验室将研究来自HIV gp 41核心的肽，这些肽被认为是HIV感染的抑制剂。 将研究这些肽和这些肽的衍生物的缔合和解离的动力学，以提供信息来帮助开发治疗AIDS的药物。 KRIEGER实验室将研究细胞表面清道夫受体，该受体结合化学修饰的脂蛋白，并与动脉粥样硬化形成、凋亡细胞和有毒及致病物质的识别和清除以及HDL代谢有关。BIACORE仪器将用于探索这些不同的多配体受体的复杂结合特性。 SAUER实验室从事以下研究：（i）阻遏物- DNA相互作用，（ii）细胞内蛋白酶如何识别靶向破坏的蛋白质中的特定序列，以及（iii）影响Arc阻遏物及其变体的同源与异源二聚化的因素。

The application by five P.I.'s proposes to use surface plasmon resonance for the kinetic and thermodynamic analysis of specific protein-protein and protein-nucleic acid interactions in a variety of biological systems. The RAJBHANDARY laboratory will perform studies of protein synthesis initiation in eubacteria and in eukaryotes. The molecular mechanisms underlying the specific interaction between the E. coli and human initiator transfer RNAs and the corresponding protein synthesis initiation factors IF2 and eIF2 will be studied using wild type and mutant initiator transfer RNAs. The KHORANA laboratory will study binding and kinetic constants of protein-protein interactions in visual processes (sensitization and desensitization) following light-activation of the vertebrate photoreceptor rhodopsin. Initial focus will be on interactions between activated receptor and the G protein, transducin, on the one hand and between the receptor and rhodopsin kinase on the other. The KIM laboratory will study peptides from the core of HIV gp41, that are known to be inhibitors of HIV infection. The kinetics of association and dissociation of these peptides, and derivatives of these peptides, will be investigated in order to provide information to assist the development of drugs to treat AIDS. The KRIEGER laboratory will study cell surface scavenger receptors which bind chemically modified lipoproteins and have been implicated in atherogenesis, in the recognition and clearance of apoptotic cells and toxic and pathogenic substances, and in HDL metabolism. BIACORE instrument will be used to explore the complex binding properties of these diverse, multiligand receptors. The SAUER laboratory is engaged in studies of (i) repressor- DNA interactions, (ii) how intracellular proteases recognize specific sequences in proteins targeted for destruction and (iii) factors that influence homo versus heterodimerization of the Arc repressor and its variants.