CORE--TISSUE ANALYSIS

组织分析和获取核心的目的将是 提供最先进的原位杂交和免疫组织化学 支持参加ESDRC的调查人员。虽然许多人 ESDRC调查人员正在进行的单独资助的研究，以及 目前在试点和可行性计划中提出的方案将使用 关于微血管内皮细胞生物学的体外模型，它 重要的是纳入解决有效性问题的实验 用活体分析进行体外观察。这一核心设施将 为分析提供专业知识、培训和设备 实验和档案组织，并将补充这项服务和 专业知识通过ESDRC的其他核心提供。组织分析与研究进展 收购核心实验室将：a)切割组织切片；b)在 原位杂交和/或免疫组织化学以更低的成本 中心成员；c)培训个人现场表演 杂交和免疫组织化学；以及d)维持组织库 包括临床标本和可供研究的实验模型 由中心教职员工使用。维护一个普通组织库将 显著加强各个实验室的工作并改善协作 在中心的参与者之间。原地加法 杂交设施将显著增强ESDRC的能力 研究人员分析实验模型并在体外解释 关于微血管内皮细胞的数据。

The purpose of the Tissue Analysis and Acquisition Core will be to provide state-of-the-art in situ hybridization and immunohistochemistry support to Investigators participating in the ESDRC. While many of the ongoing, separately funded studies by ESDRC Investigators, as well as those currently proposed in the Pilot and Feasibility Program, will use in vitro models concerning microvascular endothelial cell biology, it will be important to incorporate experiments that address the validity on in vitro observations with in vivo analysis. This Core facility will provide the expertise, training and equipment for the analysis of experimental and archival tissues, and will complement the service and expertise provide through other ESDRC Cores. The Tissue Analysis and Acquisition Core Laboratory will: a) cut tissue sections; b) perform in situ hybridization and/or immunohistochemistry at a reduced cost for members of the Center; c) train individuals to perform in situ hybridization and immunohistochemistry; and d) maintain a tissue bank consisting of clinical specimens and experimental models available for use by Center faculty. Maintenance of a common tissue bank will significantly enhance work in individual labs and improve collaborations between participants in the Center. The addition the in situ hybridization facility will significantly enhance the ability of ESDRC Investigators to analyze experimental models and interpret the in vitro data on microvascular endothelial cells.