CORE--TISSUE ANALYSIS

组织分析和采集核心的目的是 提供最先进的原位杂交和免疫组织化学 支持参与ESDRC的研究者。 虽然许多 ESDRC研究者正在进行的单独资助的研究，以及 目前在试点和可行性方案中提出的方案，将使用 涉及微血管内皮细胞生物学的体外模型，其 将重要的是纳入实验，解决有效性 体外观察与体内分析。 该核心设施将 提供专门知识、培训和设备， 实验和档案组织，并将补充服务， 通过其他ESDRC核心提供专业知识。 组织分析和 采集中心实验室将：a）切割组织切片; B）在 原位杂交和/或免疫组织化学， c）培训个人在现场表演 杂交和免疫组织化学;和d）维持组织库 包括临床标本和实验模型， 中心教师使用。 维持一个共同的组织库将 显著增强各个实验室的工作并改善协作 在中心的参与者之间。 添加原位 杂交设施将大大提高ESDRC的能力 研究人员分析实验模型并解释体外 微血管内皮细胞的数据。

The purpose of the Tissue Analysis and Acquisition Core will be to provide state-of-the-art in situ hybridization and immunohistochemistry support to Investigators participating in the ESDRC. While many of the ongoing, separately funded studies by ESDRC Investigators, as well as those currently proposed in the Pilot and Feasibility Program, will use in vitro models concerning microvascular endothelial cell biology, it will be important to incorporate experiments that address the validity on in vitro observations with in vivo analysis. This Core facility will provide the expertise, training and equipment for the analysis of experimental and archival tissues, and will complement the service and expertise provide through other ESDRC Cores. The Tissue Analysis and Acquisition Core Laboratory will: a) cut tissue sections; b) perform in situ hybridization and/or immunohistochemistry at a reduced cost for members of the Center; c) train individuals to perform in situ hybridization and immunohistochemistry; and d) maintain a tissue bank consisting of clinical specimens and experimental models available for use by Center faculty. Maintenance of a common tissue bank will significantly enhance work in individual labs and improve collaborations between participants in the Center. The addition the in situ hybridization facility will significantly enhance the ability of ESDRC Investigators to analyze experimental models and interpret the in vitro data on microvascular endothelial cells.