TREFOIL FACTORS AND ORAL MUCOSAL WOUND HEALING

三叶因子和口腔粘膜伤口愈合

• 批准号: 6100609
• 负责人: IFOR R WILLIAMS
• 金额: $ 6.87万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6100609
• 关键词: active sites; biotin; cell communication molecule; cell line; cell migration; chimeric proteins; cytoprotection; epithelium; growth factor; growth factor receptors; human tissue; in situ hybridization; oral mucosa; polymerase chain reaction; receptor binding; wound healing

Trefoil factors are a family of three small peptides secreted into the gut lumen with mucins that play a critical role in restoration of epithelial integrity following injury to gut epithelium. We propose to test the hypothesis that one or more of the trefoil factors also contribute to maintenance of epithelial integrity in the oral cavity. The specific aims of this project are: 1. To determine whether epithelial cells lining the oral cavity make any of the 3 defined human trefoil factors (pS2/TFF1, SP/TFF2, and ITF/TFF3) using a combination of RT-PCR and in situ hybridization. 2. To determine whether oral cavity epithelial cell lines can respond to recombinant intestinal trefoil factor (ITF/TFF3) with changes in their motogenic properties in a well-standardized in vitro assay for cell migration and epithelial wound healing. 3. To analyze oral cavity epithelial cell lines for the presence of, surface receptors capable of binding to ITF/TFF3 using a fusion protein that includes a consensus sequence for site-specific enzymatic biotinylation. Identification of additional natural polypeptides that favor healing of ulcerative lesions on oral mucosa is a first step towards development of new therapeutic approaches to the important clinical problems of recurrent severe apthous ulcers and chemotherapy-induced mucositis.

三叶因子是一个由三种小肽组成的家族，它们与粘蛋白一起分泌到肠腔中，在肠上皮损伤后上皮完整性的恢复中起关键作用。我们建议测试一个或多个三叶草因素也有助于的假设