DYNAMIC PROCESSES IN MYOGLOBIN

肌红蛋白的动态过程

• 批准号: 6205982
• 负责人: ETTORE BISMUTO
• 金额: $ 3.19万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6205982
• 关键词: animal tissue; biological products; biomedical resource; drug /agent

Tumor-directed drug delivery systems are being tested in mice. The use of fluorescent anti-tumor drugs has permitted evaluation of the effectiveness drug tissue targeting through the use of scanning techniques. Intensity scanning of tissue slices indicated targeting the drugs to tumor tissue but drugs were also found throughout the mouse tissue and in mouse organs. Activation of the drug induces a fluorescence lifetime change which suggested lifetime scanning as a potential method to discriminate between localization of inactive and active drug. In conjunction with ISS (Champaign, IL) a prototype scanning device was made to work with a standard ISS phase and modulation fluorometer. Preserved rat tissue slices were then scanned for intensity and fluorescence lifetime information. Phase contrast clearly indicated differences in tissues and provided preliminary evidence that lifetime imaging would be useful in discriminating drug activation from drug accumulation.

肿瘤指导的药物输送系统正在小鼠中测试。 荧光抗肿瘤药物的使用已允许评估 通过扫描的有效性药物组织靶向靶向 技术。 组织切片的强度扫描表示靶向 肿瘤组织的药物，但在整个过程中也发现了药物 小鼠组织和小鼠器官。 该药物的激活诱导A 荧光寿命的变化表明寿命扫描为 区分非活性和无效定位的潜在方法 活性药物。 与ISS（伊利诺伊州香槟）结合 扫描设备用于使用标准ISS阶段，并 调制荧光计。 然后扫描保留的大鼠组织切片 用于强度和荧光寿命信息。 相对比 清楚地表明组织的差异并提供了初步 证据表明终生成像将有用 药物积累的激活。