CONFOCAL IMAGING OF CHANGES IN CYTOPLASMIC CA2+ IN GUARD CELLS OF ARABIDOPSIS
拟南芥保卫细胞细胞质 CA2 变化的共聚焦成像
基本信息
- 批准号:6121812
- 负责人:
- 金额:$ 2.78万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1999
- 资助国家:美国
- 起止时间:1999-05-15 至 2000-04-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The elevation of cytosolic Ca2+ is a ubiquitous signaling process
in eukaryotic cells. Specificity of the physiological response
initiated by the Ca2+ signal is in part encoded by the nature of the
cytosolic Ca2+ increase. In plant cells, the cell pairs that regulate
gaseous exchange in the leaf, specificity to various stimuli (light,
temperature, abscisic acid, CO2) is thought to be encoded by spatial,
temporal and amplitude (STA) variations in the Ca2+ signal. We plan
to dissect these comples Ca2+ signals using single guard cell imaging
utilizing the techniques available at NCMIR. Previous studies in the
laboratory of Dr. J.I. Schroeder have shown that guard cells can be
loaded with the Ca2+ -sensitive dyes Calcium green and Fura 2 by a
non-invasive ester loading technique. This significant advance can
now be utilized to study Ca2+ signals in these cells. Questions such
as the source of Ca2+ (extracellular or intracellular), the STA nature
of the signal and the possible oscillation of the signal will be
addressed. The information obtained from the images obtained at NCMIR
will be combined with concurrent experiments performed in the
Schroeder laboratory that utilize electrophysiological techniques such
as patch clamping and single electrode voltage clamp. The combination
of these techniques will yield important information on the nature and
regulation of the transport processes that underlie the generation of
Ca2+ signals in guard cells. The work will use Arabidopsis thaliana
guard cells. A. thaliana mutants defective in guard cell function
have been isolated in the Schroeder laboratory. These mutants will be
tested for variations in their CA2+ signaling phenotype, so specific
mutations can be linked to defined roles in guard cell signaling. The
mutant work will focus particularly on the hormone Absisic acid (ABA).
ABA regulates guard cell turgor, and therefore, stomatal aperture by
Ca2+-mediated regulation of ion channels. Many mutants are now
available in the Schroeder laboratory which show aberrant ABA
responses. Using these ABA mutants and an integration of the
techniques available at NCMIR and in the Schroeder laboratory could
yield important information on the mechanism of action of this
important plant hormone. Work on this project is underway.
胞质Ca2+的升高是一个普遍存在的信号过程
项目成果
期刊论文数量(0)
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{{ truncateString('GETHYN J ALLEN', 18)}}的其他基金
CONFOCAL IMAGING OF CHANGES IN CYTOPLASMIC CA2+ IN GUARD CELLS OF ARABIDOPSIS
拟南芥保卫细胞细胞质 CA2 变化的共聚焦成像
- 批准号:
6282125 - 财政年份:1998
- 资助金额:
$ 2.78万 - 项目类别:
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