DISTRIBUTION OF RYANODINE RECEPTOR ISOFORMS IN CHICKEN CEREBELLUM DURING DVMT

DVMT 期间鸡小脑中兰尼碱受体异构体的分布

• 批准号: 6121835
• 负责人: YANNAN OUYANG
• 金额: $ 2.78万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-15 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121835
• 关键词: Chordata; biological products; biomedical resource; growth /development; minerals; nervous system

The ryanodine receptor is a calcium release channel localized to the internal membrane system in muscle and neurons. Three isoforms of the ryanodine receptor have been described in chicken: two skeletal muscle forms termed alpha and beta and a cardiac muscle form. The distribution of the ryanodine receptor was mapped in developing chicken brain using two monoclonal antibodies (Mab). Mab110F recognized the alpha skeletal muscle form exclusively and Mab110E recognized both the beta and cardiac forms but not the alpha form. In the developing and adult chicken brain, only cerebellar Purkinje neurons contain the alpha ryanodine receptor while neurons throughout the rest of the brain contain the beta/cardiac form. In the adult, little immunolabeling for the beta/cardiac form was seen within Purkinje neurons. However, the distribution of the beta/cardiac form changed during the first post-natal week. Whereas in the embryonic cerebellum, the beta/cardiac form was localized prim arily within Purkinje neurons, the amount of labeling within Purkinje neurons gradually decreased during the first post-natal week while a fine, punctate labeling began to appear in the molecular layer. To determine whether these profiles were indeed the presynaptic boutons of parallel fibers, 1-2 um thick sections of cerebellum were cut in the coronal orientation and examined at 400 keV using IVEM. In this orientation, the parallel fibers are running in a longitudinal direction. Using IVEM, the punctate labeling was seen to be associated with varicosites contained within longitudinal stretches of fibers of the same size and morphology as parallel fibers, providing further evidence that the beta/cardiac form is localized within presynaptic boutons of parallel fibers. This was one of the first anatomical demonstrations that nerve terminals contain calcium channels capable of mobilizing calcium from intracellular stores. This research formed part of Dr. Ouyang's doctoral dissertation. A manuscript detailing this work was published in Ouyang et al. Brain Res., 775: 52-62, 1997.

兰尼碱受体是一种钙释放通道，位于 肌肉和神经元的内部膜系统。 三种异构体 已经在鸡中描述了兰尼碱受体：两个骨骼 称为α和β的肌肉形式和心肌形式。 的 兰尼碱受体的分布被绘制在发育中 用两种单克隆抗体（Mab）对鸡脑进行了研究。 Mab110F 仅识别α骨骼肌形式，Mab 110 E 能识别β型和心形但不能识别α型 在 发育和成年鸡脑，只有小脑浦肯野 神经元含有α ryanodine受体， 大脑的其余部分包含β/心脏形式。 对于成年人来说， 几乎没有β/心脏型的免疫标记， 浦肯野神经元。 然而，β/心脏形式的分布 在产后第一周发生变化。 而在胚胎期， 小脑，β/心脏形式定位于小脑内 浦肯野神经元，浦肯野神经元内的标记量 在产后第一周逐渐减少， 分子层开始出现点状标记。 到 确定这些轮廓是否真的是突触前终扣 平行纤维，切取小脑1-2 μ m厚切片， 冠状方向，并在400 keV下使用IVEM进行检查。 在这 平行纤维在纵向上延伸， 方向 使用IVEM，观察到点状标记 与静脉曲张有关， 与平行纤维具有相同尺寸和形态的纤维， 进一步证明β/心脏型位于 平行纤维的突触前终扣。 这是最早的 神经末梢含有钙的解剖学证据 能够从细胞内储存中动员钙的通道。 这项研究是欧阳博士论文的一部分。 一 详细介绍这项工作的手稿发表在Ouyang et al. Brain上 结果：775：52-62，1997.