MEMBRANOUS COPPER AMINE OXIDASE

铜胺氧化酶（CAOs）是一个将内源性胺转化为氨、过氧化氢和醛的酶家族。迄今为止，在血清型和膜结合型两种类型的CAOs中，膜性CAOs尚未得到深入的研究。为了在哺乳动物细胞中表达该酶，并对其动力学特性、辅因子形成和催化机制进行研究，我们拟获得先前分离的膜质CAO cDNA克隆。鉴于cao在几种疾病中具有潜在的重要作用，我们打算通过组合方法合成基于肼的抑制剂。我们希望这些化合物的筛选将产生一种膜结合CAOs特异性抑制剂。我们还打算研究膜性CAO在质膜上产生H2O2的可能作用。有可能过氧化氢的原位缓慢生成将作为一种信号剂，从而影响负责细胞死亡、细胞增殖和炎症的基因。

DESCRIPTION Copper amine oxidases (CAOs) are a family of enzymes that convert endogenous amines to ammonia, hydrogen peroxide and aldehydes. To date, of t5he two types of CAOs, serum and membrane bound, the membranous CAOs have not been thoroughly studied. We intend to obtain the previously isolated clone for membranous CAO cDNA in order to express the enzyme in mammalian cells and characterize its kinetic properties, cofactor formation and catalytic mechanism. Given that CAOs are potential important in several diseases, we intend to synthesize hydrazine-based inhibitors by combinatorial methods. It is our hope that screening of these compounds will yield an inhibitor specific for membrane bound CAOs. We also intend to investigate the possible role of membranous CAO as a generator of H2O2 at the plasma membrane. It is possible that the slow, in situ generation of peroxide will act as a signaling agent, thereby influencing the genes responsible for cell death, cell proliferation and inflammation.