CONTROL OF MOUSE RETINAL CELL POPULATIONS: RODS & CONES

描述(逐字摘自申请者摘要)：长期目标 这个应用程序是为了了解如何控制 成年小鼠主要视网膜细胞群的细胞构筑。这个 这项应用的结果将描述杆和锥体的变化 背腹侧光感受器的数量、密度及其变化 不同品系的S球果、M球果和双色素球果的田间试验 确定控制这些表型的数量性状基因座(QTL)。 光感受器的数量将通过立体无偏计数获得 方法使用DIC光学技术和染色方法从视网膜整体标本中提取 冰冻切片的视网膜。锥体子类型将通过 免疫细胞化学染色。了解视网膜发育，特别是 光感受器的发育，对健康有明显的好处，因为许多人类 导致失明的疾病会影响光感受器。

DESCRIPTION (Verbatim from applicant's abstract): The long-term objective of this application is to understand the control of normal variation in the cytoarchitecture of the major retinal cell populations in adult mice. The results of this application will describe variation in rod and cone photoreceptor number and density as well as variation in the dorsoventral fields of S-, M-, and double-pigmented cones in different strains, and will identify the quantitative trait loci (QTL) controlling these phenotypes. Photoreceptor number will be acquired with stereologically unbiased counting methods from both retina wholemounts using DIC optics and from stained cryostat-sectioned retinas. Cone subtypes will be distinguished by immunocytochemical staining. Understanding retinal development, particularly photoreceptor development, has clear health benefits, because many human diseases resulting in blindness affect photoreceptors.