CONTROL OF MOUSE RETINAL CELL POPULATIONS: RODS & CONES

小鼠视网膜细胞群的控制：视杆细胞

• 批准号: 6135044
• 负责人: DAVID C AIREY
• 金额: $ 3.09万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-10 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6135044
• 关键词: cell population study; cone cell; cryostat; cytogenetics; genetic mapping; genetic regulation; genetic strain; laboratory mouse; neurogenesis; phenotype; quantitative trait loci; rod cell; visual photoreceptor

DESCRIPTION (Verbatim from applicant's abstract): The long-term objective of this application is to understand the control of normal variation in the cytoarchitecture of the major retinal cell populations in adult mice. The results of this application will describe variation in rod and cone photoreceptor number and density as well as variation in the dorsoventral fields of S-, M-, and double-pigmented cones in different strains, and will identify the quantitative trait loci (QTL) controlling these phenotypes. Photoreceptor number will be acquired with stereologically unbiased counting methods from both retina wholemounts using DIC optics and from stained cryostat-sectioned retinas. Cone subtypes will be distinguished by immunocytochemical staining. Understanding retinal development, particularly photoreceptor development, has clear health benefits, because many human diseases resulting in blindness affect photoreceptors.

描述（逐字病申请人的摘要）： 该应用是要了解对正常变化的控制 成年小鼠主要视网膜细胞种群的细胞结构。这 该应用的结果将描述杆和锥体的变化 感光体数量和密度以及背腹的变化 不同菌株中的S-，M-和双重色锥的场，并将 确定控制这些表型的定量性状基因座（QTL）。 光感受器的数量将通过立体理学无偏的计数获取 使用DIC光学元件和染色的视网膜全段的方法 低温恒温器截面视网膜。锥亚型将通过 免疫细胞化学染色。了解视网膜发展，特别是 感光受体开发，具有明显的健康益处，因为许多人 导致失明的疾病会影响光感受器。

项目成果

Blood pressure, vessel caliber, and retinal thickness in diabetes.

• DOI: 10.1097/opx.0b013e3182775c62
• 发表时间: 2012-12
• 期刊: Optometry and vision science : official publication of the American Academy of Optometry
• 作者: Harrison WW;Chang A;Cardenas MG;Bearse MA Jr;Schneck ME;Barez S;Adams AJ
• 通讯作者: Adams AJ