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MOLECULAR CLONING AND EXPRESSION OF MIDKINE RECEPTOR

中期因子受体的分子克隆和表达

基本信息

批准号：
6164498
负责人：
LIBO QIU
金额：
$ 4.63万
依托单位：
ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-03-01 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/6164498
关键词：
3T3 cells biological signal transduction cell growth regulation growth factor receptors in situ hybridization kidney molecular cloning receptor expression transfection vascular endothelial growth factors

项目摘要

The long term aim of the proposed research is to define the molecular structure and function of the midkine signal transduction pathway which regulates cellular proliferation and differentiation Special emphasis in this effort is placed on the molecular cloning of the cell surface Midkine signal transduction receptor which we have recently identify from a Wilms~ tumor cell line using biochemical techniques. Recent studies have established the importance of Midkine expression in Wilms~ tumor, renal cell carcinoma and lung, colon and hepatocellular carcinoma. Our studies have also shown Midkine expression in autosomal dominant polycystic kidney disease (ADPKD) and its role as an important mediator in epithelial differentiation during kidney organogenesis. Our hypothesis is that Midkine functions as an autocrine or paracrine which regulates several biological processes including the growth regulation of epithelial cells in ADPKD as well as in oncogenic transformation in Wilms~ tumor and in differential mechanisms which regulate epithelial conversion of renal progenitor cells during renal development. We plan to clone Midkine receptor gene using either an expression cloning approach or ligand affinity chromatography to purify the MK receptor protein. Additionally, we also plan to examine Midkine signal transduction pathway, then define the basic developmental pattern in the kidney of expression of all identified components of the midkine signal transduction pathway including expression of the Midkine receptor in relation to its ligand.

这项研究的长期目标是确定分子

项目成果

期刊论文数量（2）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Midkine promotes selective expansion of the nephrogenic mesenchyme during kidney organogenesis.

中期因子在肾脏器官发生过程中促进肾源性间充质的选择性扩张。

DOI：
10.4161/org.1.1.979
发表时间：
2004
期刊：
Organogenesis
影响因子：
2.3
作者：
Qiu,Libo;Hyink,DeborahP;Gans,WilliamH;Amsler,Kurt;Wilson,PatriciaD;Burrow,ChristopherR
通讯作者：
Burrow,ChristopherR

Monomeric midkine induces tumor cell proliferation in the absence of cell-surface proteoglycan binding.

单体中期因子在没有细胞表面蛋白聚糖结合的情况下诱导肿瘤细胞增殖。