CORE--TISSUE CULTURE/MEDIA PREPARATION

核心——组织培养/培养基准备

• 批准号: 6203013
• 负责人: VINCENT T DEVITA
• 金额: --
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-12 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6203013
• 关键词: biomedical facility; cell bank /registry; cryopreservation; growth media; monoclonal antibody; neoplastic cell; tissue /cell culture

The functions of the Tissue Culture/Media Preparation Shared Resource are to: a) prepare required solutions; 2) supply sterile glassware for tissue culture users; c) maintain stock cultures and periodically monitor them for contamination; e) maintain cultured cells in a frozen state; f) ensure the proper functioning of required instrumentation, such as the Coulter Counter, autoclaves, CO2 incubators, Biogard hoods and microscopes; g) provide technical assistance and advice when needed; h) cryopreserve culture cells using a Cryomed 1010 Controller i) detect mycoplasma using a Hoechst stain assay; j) production of monoclonal antibodies; k) filtration and distribution of a variety of low cost media free from mycoplasma and other contaminants.

组织培养/培养基制备共享资源的功能是 以：a）制备所需溶液; 2）提供组织用无菌玻璃器皿 培养物使用者; c）保持储备培养物并定期监测 用于污染; e）将培养的细胞保持在冷冻状态; f）确保 所需仪器的正常运行，例如Coulter 计数器、高压灭菌器、CO2培养箱、Biogard罩和显微镜; g） 在需要时提供技术援助和咨询; h）冷冻保存 使用Cryomed 1010控制器培养细胞i）使用 a Hoechst染色测定; j）单克隆抗体的产生; k） 过滤和分配各种低成本介质， 支原体和其它污染物。