CORE--TISSUE CULTURE/MEDIA PREPARATION

核心——组织培养/培养基准备

• 批准号: 6101778
• 负责人: VINCENT T DEVITA
• 金额: --
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-23 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6101778
• 关键词: biomedical facility; cell bank /registry; cryopreservation; growth media; monoclonal antibody; neoplastic cell; tissue /cell culture

The functions of the Tissue Culture/Media Preparation Shared Resource are to: a) prepare required solutions; 2) supply sterile glassware for tissue culture users; c) maintain stock cultures and periodically monitor them for contamination; e) maintain cultured cells in a frozen state; f) ensure the proper functioning of required instrumentation, such as the Coulter Counter, autoclaves, CO2 incubators, Biogard hoods and microscopes; g) provide technical assistance and advice when needed; h) cryopreserve culture cells using a Cryomed 1010 Controller i) detect mycoplasma using a Hoechst stain assay; j) production of monoclonal antibodies; k) filtration and distribution of a variety of low cost media free from mycoplasma and other contaminants.

组织培养/培养基制备共享资源的功能如下 A)准备所需的溶液；2)提供无菌纸巾用玻璃器皿 文化用户；c)维护库存文化并定期监测 对于污染；e)将培养的细胞保持在冰冻状态；f)确保 所需仪器的正常运行，如库尔特 计数器、高压灭菌器、二氧化碳培养箱、生物加热罩和显微镜；g) 在需要时提供技术援助和咨询；h)低温保存 使用CRYOMED 1010控制器的培养细胞I)检测支原体 Hoechst染色试验；j)生产单抗；k) 各种低成本介质的过滤和分配 支原体和其他污染物。